Determination of carbamazepine concentration in human saliva by HPLC and its pharmacokinetic study

    TANG Zhe,WEN Aiª²Dong,WU Yin, ZHAO Lei, LI Hongª²Li, SHI Ru

    Department of Pharmacy,Xijing Hospital,Fourth Military Medical University, Xi¬ðan 710033, China

    ¡¾Abstract¡¿ AIM: To determine carbamazepine(CBZ) concentration in human saliva by HPLC and study its pharmacokinetics. METHODS: Shimª²pack CLCª²ODS C18 (150 mm¡Á6 mm, 5 ¦Ìm) spectrum analytical column was used and methanolª²water(53¡Ã47)was adopted as the mobile phase. Flow rate was 1.0 mL¡¤min-1, detection wavelength was 212 nm, column temperature was 23¡æ and the dosage of CBZ was 50 ¦ÌL. The pharmacokinetic parameters of CBZ tablets taken by 6 healthy volunteers at a single oral dose of 200 mg were calculated according to CBZ concentration in saliva. RESULTS: The standard curve was linear (r=0.999) within the range of 0.40~4.32 mg¡¤L-1 for CBZ. The limit of detection in saliva was 30 ¦Ìg¡¤L-1 (S/N¡Ý3). The average recovery was 99.17%-104.80%£»the interª²day RSD and intraª²day RSD was 2.6%-4.9% and 3.0%-8.6% respectively.The results showed that the pharmacokinetics of CBZ was in accordance with the oneª²compartment open model. Tmax, Cmax, ke, T1/2 and AUC(0~inf) was (4.50¡À3.15) h, (1.52¡À0.22) mg¡¤L-1, (0.0090¡À0.0006) h-1, (77.16¡À5.26) h and (132.18¡À8.82) mg ¡¤h¡¤L-1 respectively. After single dosing, the concentrationª²time curve of CBZ in saliva presented multiple peaks. CONCLUSION: This method is simple, quick and specific, and can be used to determine CBZ concentration in human saliva and to study its pharmacokinetics. ......