关键词：人膜联蛋白；乙型肝炎病毒；免疫组织化学；胎儿

    摘要：目的 观察HBV感染相关因子——人膜联蛋白(Human Annexin V，HA-V)在胎儿各组织中的表达。方法 用SP免疫组化法检测HA-V在胎儿肝脏、心脏、肾脏、卵巢、输卵管、脾脏、胸腺中的表达及分布。结果 肝脏、肝内胆管系统、心脏、肾脏、卵巢、脾脏、输卵管、胸腺中均有HA-V表达，HA-V分布于这些组织的实质细胞及间质细胞的胞质；免疫组化图像分析系统显示胎儿肝脏组织中HA-Ⅴ表达显著多于其他组织(P<0.05)，其余组织之间无明显差别(P>0.05)。 结论 HA-Ⅴ是人体组织中的一种固有蛋白，分布于多种组织中，这种分布的广泛性可能与HBV泛嗜性感染相关，而肝脏组织中HA-Ⅴ表达明显多于其他组织可能是HBV主要侵犯肝组织的重要原因之一。

    Expression of human annexin V in different fetal tissues

    YE feng, LI Shu-hong, CHEN Tian-yan, YUE Ya-fei, ZHANG Shu-lin, BAI Gui-qin

    Department of Infectious Disease, First Hospital of Xi’an Jiaotong University, Xi’an 710061, China

    Abstract: Objective To investigate the expression of human annexin-V (HA-V) in relation to HBV infection in different fetal tissues. Methods Immunohistochemistry was employed to detect the expression and distribution of HA-V in the liver, kidney, ovary, heart, fallopian tube, spleen, and thymus gland of human fetus. Results HA-V expression was detected in different tissues including the ovary, liver, intrahepatic bile duct, heart, kidney, lymphocytic cells in the thymus gland, epithelial cells of the fallopian, and cortical and medullary cells of the spleen. HA-V was distributed mainly in the cytoplasm of the cells. The liver tissues exhibited greater gray scale for HA-V expression than in the other tissues (P<0.05) and no significant difference was observed in the other tissues than the liver (P>0.05) in image analysis with Photoshop 7.0. Conclusion HA-V is an inherent protein in fetal tissues with possible relation to HBV infection of different tissues as a HBV receptor. Greater amount of HA-V in the liver may account for the vulnerability of the liver to HBV infection. ......