Caveolin-1对胃癌细胞系MGC803细胞生长的影响
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罗红梅, 唐圣松, 廖端芳, 严鹏科, 谭力铭,
Caveolin-1;胃癌;细胞增殖;分化;基因治疗,罗红梅,龙治峰,刘月顺,唐圣松,廖端芳,严鹏科,谭力铭,汪煜华,朱炳阳,罗红梅,通讯作
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罗红梅, 龙治峰, 刘月顺, 南华大学医学院组胚教研室 湖南省衡阳市 421001
唐圣松, 廖端芳, 严鹏科, 谭力铭, 汪煜华, 朱炳阳, 南华大学药物药理研究所 湖南省衡阳市 421001
罗红梅, 2004年南华大学病理学与病理生理学硕士, 讲师, 2005年南华大学博士研究生, 主要从事分子肿瘤学的研究.
国家自然科学基金资助项目, No. 30270684
通讯作者: 唐圣松, 421001, 湖南省衡阳市, 南华大学医学院药物药理研究所. tangss111@yahoo.com.cn
电话: 0734-8282854 传真: 0734-8281305
收稿日期: 2006-03-07 接受日期: 2006-03-29
Effect of Caveolin-1 on growth of human gastric cancer cell Line MGC803
Hong-Mei Luo, Sheng-Song Tang, Duan-Fang Liao, Peng-Ke Yan, Li-Ming Tan, Yu-Hua Wang, Zhi-Feng Long, Yue-Shun Liu, Bing-Yang Zhu
Hong-Mei Luo, Zhi-Feng Long, Yue-Shun Liu, Department of Histology and Embryology, Medicial College of Nanhua University, Hengyang 421001, Hunan Province, China
Sheng-Song Tang, Duan-Fang Liao, Peng-Ke Yan, Li-Ming Tan, Yu-Hua Wang, Bing-Yang Zhu, Institute of Pharmacy and Pharmacology, Medicial College of Nanhua University, Hengyang 421001, Hunan Province, China
Supported by National Natural Science Foundation of China, No. 30270684
Correspondence to: Dr. Sheng-Song Tang, Institute of Pharmacy and Pharmacology, Medicial College of Nanhua University, Hengyang 421001 Hunan Province, China. tangss111@yahoo.com.cn
Received: 2006-03-07 Accepted: 2006-03-29
Abstract
AIM: To investigate the effect of Caveolin-1 on the proliferation and differentiation of gastric carcinoma cell line MGC803, and explore the probability that Caveolin-1 can be used for gene therapy.
METHODS: Caveolin-1 gene and Pcl-neo control plasmid were transfected into human MGC803 cell line by lipofectin, respectively. The positive clones were selected by G418. We also stabled a positive control group which was treated with PD98059 for 48 hours. Then the expression of Caveolin-1 in each group was detected by Western blot. Cell morphology was observed under optical microscope. Cell population doubling time was determined by cell counting method and cell cycle was analyzed by flow cytometry ......
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