高血压__(期刊论文)

L精氨酸对肾性高血压大鼠心血管组织RAS的影响

http://www.100md.com 《咸宁学院学报(医学版)》 2005年第6期

高血压,,高血压；主动脉；血管肥厚；肾素血管紧张素系统；L精氨酸,1材料与方法,2结果,3讨论,参考文献：

     摘要：目的探讨一氧化氮前体L精氨酸对肾血管性高血压大鼠心血管组织局部肾素血管紧张素系统(RAS)的影响。方法采用两肾一夹(2K1C)型肾血管性高血压大鼠模型。所有大鼠被随机分为4组(每组10只)：①假手术对照组；②高血压对照组；③L精氨酸治疗组，于术后第5周开始给予L精氨酸200 mg·kg-1·d-1；④LNAME处理组，于术后第5周开始同时给予LNAME 10 mg·kg-1·d-1及L精氨酸200 mg·kg-1·d-1。采用标准尾套法间接检测清醒大鼠血压。给药8周后，大鼠处死，分离心脏及主动脉用于检测组织血管紧张素Ⅱ含量(放射免疫法检测)和血管紧张素转换酶活性(分光光度计法检测)。结果与假手术对照组大鼠相比，未给药2K1C高血压大鼠血压明显升高，心脏及主动脉的血管紧张素Ⅱ(AngⅡ)含量增加和血管紧张素转换酶(ACE)活性增高。应用L精氨酸治疗则明显抑制了肾动脉狭窄术后大鼠血压的升高，降低了2K1C高血压大鼠心脏及主动脉的AngⅡ含量和ACE活性。NOS抑制剂LNAME明显抑制了L精氨酸的上述作用。结论长疗程L精氨酸降压机制可能与抑制心血管组织局部RAS有关。

    关键词：高血压；主动脉；血管肥厚；肾素血管紧张素系统；L精氨酸

    The Effects of Larginine on ReninAngiotensin System in Cardiovascular Tissue of Rats with Renovascular Hypertension

    XU Jinzhang,LIU Shouxian

    (Xianning Office of Drugassay,Xianning Hubei 437100,China)

    ABSTRACT:Objective To investigate the role of Larginine on reninangiotensin system(RAS) in cardiovascular tissue of rats with renovascular hypertension. Methods The renovascular hypertension model was induced in rats by twokidneyoneclip(2K1C) method. All the rats were randomly divided into four groups (n=10 per group): ①the shamoperated control group;②the 2K1C renohypertension control group; ③the LArg group, the 2K1C rats were treated with Larginine at a dose of 200mg·kg-1·d-1 from 5th week after renal artery constriction; ④the LNAME (Nitroarginine methyl ester) group, the 2K1C rats were treated with LNAME (10 mg·kg-1·d-1) plus Larginine (200mg·kg-1·d-1) from 5th week after renal artery constriction. Indirect blood pressure was measured in conscious rats by the standard tailcuff methods. After treatment with both drugs for 8 weeks, the rats were killed and the heart and aorta were removed to determine angiotensin Ⅱ content by radioimmunoassay and angiotensinconverting enzyme activity by spectrophotometric method. Results Compared with the shamoperated control group, the untreated 2K1C renohypertensive rats exhibited an increase in blood pressure, aortic angiotensin Ⅱ content and angiotensinconverting enzyme activity of the heart and aorta. Larginine significantly inhibited the increase in blood pressure, aortic angiotensin Ⅱ content and angiotensinconverting enzyme activity of the heart and aorta in 2K1C renovascular hypertensive rats; and these effects of Larginine could be inhibited by LNAME. Conclusion Longterm treatment with Larginine could lower blood pressure in renovascular hypertensive rats, which might be related to its inhibition on local tissue reninangiotensin system. ......

百拇医药网 http://www.100md.com/html/DirDu/2006/05/31/97/97/69.htm

您现在查看是摘要页，全文长 8099 字符。