[摘 要] 目的：研究体外培养的肺泡Ⅱ型细胞(AECⅡ)VEGF，Flt-1，Flk-1，SP-B的表达。方法：体外培养AECⅡ，采用免疫组织化学方法和电镜鉴定细胞，然后应用SABC法检测AECⅡ 的VEGF,Flt-1,Flk-1, SP-B 的表达。结果：AECⅡ培养72 h，Flt-1，Flk-1，VEGF阳性表达，SP-B阴性表达。结论：体外培养的AECⅡ，表达Flt-1、Flk-1等受体，人工干预应用VEGF可能发生一系列信号转导，为医学研究应用VEGF提供理论依据。

    [关键词] 肺泡Ⅱ型上皮细胞；细胞培养；受体；Flk-1；Flt-1

    Flk-1 receptor expression in primary culture of type two alveolar epithelial cells

    XIAO Yu,LI Tao,XIE Ji-jian.

    (Department of Pediatrics, Taihe Hospital, Yunyang Medical College, Shiyan, Hubei 442000, China)

    Abstract:Objective To investigate the expression of VEGF, Flt-1, Flk-1 and SP-B in primary culture of type two alveolar epithelial cell (AECⅡ). Methods AECⅡ was cultured in vitro and characterized with immunohistochemistry and electronic microscopy. The expression of VEGF, Flt-1, Flk-1 and SP-B was detected with SABC method.Results The expression of Flt-1,Flk-1 and VEGF was observed, the SP-B expression was negative.Conclusion Cultured AECⅡ in vitro expresses Flt-1,Flk-1. Exogenous administration of VEGF to AECⅡ may trigger a series of signal transduction and this study provides a basis clinical application of VEGF. ......