高效液相色谱法__(期刊论文)

高效液相色谱法测定黄毛耳草中槲皮素的含量

http://www.100md.com 《广东药学院学报》 2006年第1期

高效液相色谱法,,黄毛耳草;槲皮素;含量测定;高效液相色谱法,1仪器与试药,2色谱条件及系统适应试验,3方法学考察,4讨论,参考文献:

     摘要:目的建立黄毛耳草中槲皮素含量的测定方法。方法色谱柱:ODS Kromasil C18色谱柱(4.6 mm×150 mm，5 μm)；柱温：35 ℃;流动相：甲醇0. 4 %磷酸 (体积比45∶55),流速：1.0 mL/min,检测波长：365 nm。结果槲皮素进样量在0.005～0.045 μg 范围内与峰面积线性关系良好(r=0.9997),回收率99.88 %,RSD 0.58 %。结论本法简便,结果可靠，可用于黄毛耳草及其制剂的质量控制。

    关键词:黄毛耳草;槲皮素;含量测定;高效液相色谱法

    HPLC for content measurement of quercetin in Hedyotis chrysoticha

    Li Ping

    (Wuhu Institute for Drug Control,Wuhu 241001,China)Abstract: Objective To establish an HPLC method for content determination of quercetin in Hedyotis chrysoticha (Palib.) Merr. Methods HPLC method was carried on Kromasil C18 column using methanol0.4% phosphoric acid (45∶55) as the mobile phase under the detection wavelength of 365 nm.Results The calibration curve for quercetin was linear in the range of 0.005-0.045μg. The average recovery was 99.88 % with RSD 0.58 %. Conclusions The method is simple,convenient and reliable for quality control of Hedyotis chrysoticha (Palib.) Merr.

    Key words:Hedyotis chrysoticha; HPLC; quercetin

    黄毛耳草为茜草科植物黄毛耳草Hedyotis chrysoticha (Palib.) Merr.的干燥或新鲜全草 ......

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