水牛角与羚羊角的HPLC法鉴别
水牛角,,高效液相色谱法;水牛角;羚羊角,1仪器和试剂,2试验方法,3结果,4小结与讨论
摘 要 建立一种鉴别水牛角与羚羊角药材的质控方法。采用OPA柱前衍生反相高效液相色谱法，色谱柱为HP Hypersil ODS柱(125 mm×4.6 mm×5μm)，以十八烷基硅烷键合硅胶为填充剂。流动相A：8.0 mmol/L磷酸盐缓冲液(pH7.2)，含φ=0.3%四氢呋喃；流动相B：8.0 mmol/L磷酸盐缓冲液(pH7.2)+甲醇+乙腈(50+35+15)。线性梯度洗脱；荧光检测器,Ex=340 nm，Em=450 nm。结果水牛角与羚羊角药材在氨基酸的含量上有明显差异。本文所建立的方法简便，重现性好，可用于水牛角与羚羊角药材的鉴别。关键词 高效液相色谱法;水牛角;羚羊角
中图号 R282.5 文章编号:1006-8783(2003)02-0097-02 文献标识码:A
The differentiation of Bubalus bubalis Linnaeus. from Saiga tatarica Linnaeus. by HPLC
LI Xiaomeng，HE Xinrong
(1.Department of Natural Pharmaceutical Chemistry,2.Department of Pharmacognosy,Guangdong College of Pharmacy,Guangzhou 510224)
Abstract An differentiation method of Bubalus bubalis Linnaeus. from Saiga tatarica Linnaeus. by HPLC was established . HP Hypersil ODS column was used with octadecyl silane boned silica gel as filler. Mobile A consisted of 8.0 mmol/L potassium(pH7.2) and 0.3% tetrahydrofuran;mobile B consisted of 8.0 mmol/L potassium(pH7.2),MeOH and acetonitrile(50∶35∶15). A Fluorescence detector was adopted with Ex=340 nm ......
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