桑黄_____桑黄；药用真菌；液体发酵；菌丝产量

药用真菌桑黄液体发酵工艺的研究

http://www.100md.com 《广东药学院学报》 2004年第3期

桑黄,,桑黄；药用真菌；液体发酵；菌丝产量,1材料与方法,2结果与分析,3讨论,参考文献

     摘要目的：研究不同培养基、不同培养条件对桑黄菌丝生长的影响。方法：通过液体发酵培养，并利用L9(34)正交试验筛选出了桑黄液体发酵的优化培养基。结果与结论：玉米粉为最佳碳源，麸皮为最佳氮源，优化培养基配方为：玉米粉5%、麸皮3%、磷酸二氢钾0.3%、硫酸镁0.15%、维生素B1 20μg/100 mL、维生素B2 30μg/100 mL；最适菌丝体生长的液体发酵条件为：培养温度为26 ℃，摇瓶转速130 r/min，pH值6.5，接种量15%~20%，种子液培养时间为5 d，装液量(500 mL三角瓶)为140 mL，发酵时间为7 d。

    关键词桑黄；药用真菌；液体发酵；菌丝产量

    Study on liquid fermentation technology of medicative fungus phellinus igniarius

    YANG Quan,LI Yanhui,YAN Hanjing,WANG Qi

    (1. Section of Medical Plant and Pharmacognosy, Guangdong College of Pharmacy, Guangzhou Guangdong 510240;

    2. Chendu Institute of Biology, Chinese Academy of Sciences, Chendu,Sichuan 610041; 3. Faculty of Traditional Chinese Medicinal Crops, Jilin Agricultural University, Changchun,Jilin 130118)

    Abstract AbstractTo study the effects on mycelia by various media and conditions, including shaking culture and L9(34)orthogonal test. The result showed that corn flourwas the optimalcarbon source andbran is the optimalnitrogen source.The optimalculture mediumwas: corn flour 5%，bran 3%,KH2PO4 0.3%,MgSO4 0.15%,VitB120 μg/100 mL,VitB230 μg/100 mL.The optimalfermented condition was: culture temperature 26 ℃ ......

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