抗加特纳杆菌卵黄免疫球蛋白(IgY)的研究
阴道加特纳杆菌,,阴道加特纳杆菌;卵黄免疫球蛋白(IgY);多克隆抗体,1材料和方法,2结果与分析,3讨论,参考文献
摘 要 目的 观察鸡卵黄中抗加特纳杆菌抗体的产量、纯度、来源及稳定性。方法 用阴道加特纳杆菌作为抗原免疫Leghorn鸡。通过改良水稀释法提取卵黄中的IgY。双紫外光波长测定抗体含量，SDS-PAGE电泳检测抗体纯度。Western blot 免疫印迹法测定该抗体来源。ELISA检测IgY对温度、酸碱度的稳定性。结果 抗体含量12.8 mg/mL蛋黄液，抗体纯度达95%。免疫印迹证明IgY与鸡血清中的IgG具有相同的分子量和抗原性。IgY具有良好的热稳定性，对酸碱具有一定的耐受力。结论 WD水稀释法能得到高产量、高纯度的特异性IgY，而且有良好的生物学活性。关键词 阴道加特纳杆菌;卵黄免疫球蛋白(IgY);多克隆抗体
Studies of Immunoglobulin of yolk (IgY) against Gardnerella
LIN Yuanzao , HUANG Qingsong, LI Chunmei, ZHOU Yan
(1.Section of Biochemistry, Guangdong College of Pharmacy, Guangzhou,Guangdong 510240; 2. Guangzhou Xinhai Hospital, Guangzhou,Guangdong 510303)
Abstract Objective to observe the yield, purity and stability of IgY against Gardnerella. Methods laying hens (White Leghorn) were immunized with G. Vaginalis. IgY was purified with the modified simple water dilution method (WD) and its purity and yield were measured respectively with SDSPAGE and ultraviolet spectrophotometry. The antigenicity of IgY anainst heating, acid and alkali, repeated freezing and fusion treatments were researched with enzymelinked immunoadsorbent assay (ELISA) technology. Results purity of IgY was about 95% and 12.8 mg IgY from per milliliter egg yolk was routinely obtained. Moreover, IgY had the same molecular weight and antigenicity in comparison with IgG of chick serum. The IgY was fairly heatstable and had a certain degree of acidalkali resistance. Conclusion high yield and purity of specific antibody IgY from egg yolk was obtained successfully by WD purification methods, and it showed good biological activities. ......
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