大孔树脂富集纯化岗梅根中总皂苷的研究
大孔树脂,,大孔树脂;,岗梅根总皂苷;,纯化,1仪器与试药,2方法与结果,3讨论,参考文献
摘 要 目的：研究大孔树脂富集、纯化岗梅根总皂苷的工艺条件及参数。方法：以岗梅根总皂苷的洗脱率和精制度为指标考察了大孔树脂富集、纯化岗梅根总皂苷的吸附性能和洗脱参数。结果：岗梅根提取液47.3 mL上柱，依次用100 mL蒸馏水洗去水溶性杂质，100 mL 70%乙醇洗脱总皂苷，总皂苷富集在70%的部分，且去除杂质的能力强。结论：通过大孔树脂富集与纯化后的总皂苷洗脱率在90%以上，纯化后总固型物中岗梅根总皂苷含量为62.98%。采用此法可以较好地纯化岗梅根总皂苷。关键词 大孔树脂; 岗梅根总皂苷; 纯化
Study on purification process of total saponin of radix ilicis asprellae with macroreticular resin
FENG Daxing,YU Chuqin,ZHONG Ming
(1.TCM Hospital of BaiYun District, Guangzhou,Guangdong 510450; 2.Institute of materia Medica, Guangdong College of Pharmacy, Guangzhou,Guangdong 510240; 3.GuangZhou Hangfang Natural Medicine Research & Development Co.,Ltd. Guangzhou,Guangdong 510240)
Abstract Objective: to study the technological conditions and parameters of the purification process of total saponin of Radix Ilicis Asprellae with macroreticular resin. Methods: the adsorptive characteristics and elusive parameters of the process were studied by taking the elusive and purified ratio of total saponin as reference. Results: 47.5 mL of the extractive of Radix Ilicis Asprellae was purified with a column of macroreticular resin and washed with 100 mL of distilled water, then eluted with 100 mL of 70% ethanol. Conclusions: with macroreticular resin to adsorb and purify, the elusive ratio of total saponin was above 90% and the purity reached 62.98%. So theapplying of macroreticular resin to adsorb and purify total saponin of Radix Ilicis Asprellae is feasible. ......
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