肾__(论著)

缺血再灌注肾血管反应性损伤中的作用

http://www.100md.com 《现代医学》 2006年第2期

肾,,]肾；缺血再灌注损伤；离体灌流肾；过氧亚硝基阴离子；氨基胍；肾血管反应性；大鼠,1材料与方法,2结果,3讨论,[参考文献]

     [摘要]目的探讨过氧亚硝基阴离子(ONOO-)在大鼠缺血再灌注肾血管反应性损伤中的作用。方法应用离体灌流肾(isolated perfused kidney, IPK)技术观察肾缺血再灌注(ischemia reperfusion，IR)后肾血管对去甲肾上腺素(norepinephrine，NE)和乙酰胆碱(acetylcholine，Ach)反应性的改变；观察应用氨基胍(aminoguanidine，AG)阻断内源性NO生成对肾IR大鼠IPK肾血管反应性的影响，以及给予外源性ONOO-后大鼠IPK肾血管反应性的改变。结果 IR后IPK肾血管对NE的缩血管反应性和对Ach的舒血管反应性都有明显降低，IR5h较IR1h进一步降低(均P<0.01)。ONOO-(40μmol·L-1)与vehicle相比则显著降低IPK肾血管对NE和Ach的反应性(均P<0.01)。AG+IR1h组大鼠IPK肾血管对NE和Ach的反应性与NS(生理盐水)+IR1h组大鼠相比无显著差异(均P>0.05)，AG+IR5h组大鼠肾血管对NE和Ach的反应性较NS+IR5h组明显增强(均P<0.05)。结论 ONOO-在肾IR所致的肾血管反应性损伤中具有重要的作用。

    [关键词]肾；缺血再灌注损伤；离体灌流肾；过氧亚硝基阴离子；氨基胍；肾血管反应性；大鼠

    Effects of peroxynitrite on renal vascular response in isolated

    perfused kidney after rats renal ischemia reperfusionWANG Xinliang1, DUAN Guowei 2, XUE Xiaoying2,LI Hongjuan1

    (1. Department of Pediatrics, the Second Hospital, Hebei Medical University, Shijiazhuang 050000, China; 2. Department

    of Radiotherapy, the Second Hospital, Hebei Medical University, Shijiazhuang 050000, China) Abstract：Objective To investigate the effects of peroxynitrite(ONOO-) on renal vascular response in isolated perfused kidney(IPK) after renal ischemia reperfusion(IR). Methods IPK was used to examine the effects of renal IR on renal vascular response, the effects of iNOS inhibition on the renal vascular response changes caused by renal IR, and the effects of administration of ONOO- on above parameters. Results Contractile response of renal vascular to norepinephrine(NE) and relaxation responses of renal vascular to acetylcholine(Ach) both were depressed obviously, IR5h was overdepressed compared with IR1h (all P<0.01). ONOO-(40μmol·L-1)depressed notably the renal vascular response to NE and Ach compared with vehicle (all P<0.01). The renal vascular response to Ach and NE was not effected in IR1h by AG, but was strengthened in IR5h (all P<0.05). Conclusion it is proved in IPK model that the generation of ONOO- possesses important role in renal vascular response damage of rats after renal IR. ......

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