慢病毒_____RNA干扰；Smad4；肿瘤；慢病毒

Smad4基因RNAi慢病毒载体的构建与鉴定

http://www.100md.com 《第四军医大学学报》 2006年第7期

慢病毒,,RNA干扰；Smad4；肿瘤；慢病毒,0引言,1材料和方法,2结果,3讨论,【参考文献】

     Construction and identification of lentiviral vector of RNA interference of Smad4 gene

    JI GuoZhong, ZHANG FaMing, ZHAI HuiHong, FAN ZhiNing, FAN DaiMing, WANG XueHao

    Department of Gastroenterology, Second Affiliated Hospital, Nanjing Medical University, Nanjing 210011, China， Institute of Gastrointestinal Diseases，Xijing Hospital，Fourth Military Medical University，Xian 710033, China， Department of Hepatobiliary Surgery, First Affiliated Hospital, Nanjing Medical University, Nanjing 210029, China

    【Abstract】 AIM: To construct a lentiviral vector of RNA interference (RNAi) of Smad4 gene. METHODS: The effective sequence of siRNA targeting Smad4 gene was confirmed in our previous study. The complementary DNA containing both sense and antisense Oligo DNA of the targeting sequence was designed, synthesized and cloned into the pLVTHM vector, which contained H1 promoter and green fluorescent protein (GFP). The resulting lentiviral vector containing Smad4 shRNA was named LVshSmad4, and it was confirmed by PCR and sequencing. 293T cells were cotransfected with lentiviral vector LVshSmad4 ......

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