原核表达_____CD40L;_克隆;

人可溶性CD40L cDNA的克隆及表达

http://www.100md.com 《第四军医大学学报》 2006年第7期

原核表达,,CD40L;,克隆;,原核表达,人可溶性CD40LcDNA的克隆及表达,0引言,1材料和方法,2结果,3讨论,【参考文献】

     Cloning of human sCD40L cDNA and construction of vector for expression in E. coli

    ZHAO ZhenGuo，JIANG Yu，PENG JiaHe

    Department of Biochemistry and Molecule Biology, Third Military Medical University, Chongqing 400038，China

    【Abstract】 AIM: To clone human sCD40L(184-831) cDNA and construct a prokaryotic vector for expression in E. coli. METHODS: The human sCD40L(184-831 ) cDNA was amplified with the total RNA from the human tonsil by reverse transcriptase (RT)PCR, and was analyzed by enzymatic digestion and sequencing. Then, the insert of human sCD40L(184-831) cDNA was cloned into the vector pET28a+ for expression in BL21, and then the interest protein was identified by Western blot. RESULTS: The result of sequencing was accordant with the one reported. The prokaryotic expression vector was constructed successfully and the interest protein was expressed in BL21. CONCLUSION: The human sCD40L(184-831) cDNA has been successfully cloned and a prokaryotic system been constructed for expression. The interest protein was expressed successfully in BL21. ......

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