花剥苔;_____]_花剥苔;_哮喘;

小儿哮喘花剥苔产生的免疫机制研究

http://www.100md.com 《中西医结合学报》 2005年第6期

花剥苔;,,],花剥苔;,哮喘;,免疫,1资料与方法,2结果,3讨论,[参考文献]

     [摘要] 目的：从免疫学角度探讨哮喘患儿花剥苔形成的机制。方法：采用免疫组化方法检测舌苔脱落上皮细胞中角蛋白13及bcl2蛋白的含量。应用流式细胞术检测外周血CD4+及细胞趋化因子受体3(cell chemokine receptor3, CCR3)的表达量。采用放射免疫法检测血清中皮质醇的含量。结果：哮喘花剥苔组患儿外周血CD4+和CCR3水平明显高于哮喘非花剥苔组及正常组(P<0.05)；哮喘花剥苔组、非哮喘花剥苔组血清中皮质醇含量低于其余各组(P<0.05)。哮喘花剥苔、非花剥苔组血清IgE水平明显高于其余各组(P<0.05)。哮喘花剥苔组、非哮喘花剥苔组舌苔上皮脱落细胞中角蛋白13的含量明显低于哮喘非花剥苔组、非哮喘非花剥苔组和正常对照组(P<0.05)。哮喘花剥苔组患儿舌苔上皮脱落细胞中bcl2蛋白含量与其余各组比较无统计学差异。结论：花剥苔的形成与免疫反应密切相关，血清皮质醇水平降低、CD4+及CCR3增多，可能促进了花剥苔的形成。同时，舌上皮细胞角化障碍及舌上皮细胞凋亡亦可能促进花剥苔的形成。

    [关键词] 花剥苔; 哮喘; 免疫

    Immunological mechanism of exfoliative tongue fur in children with asthma

    LI FuFeng, LI GuoGang, WU YouZhong, LI Jing, ZHANG XiaoYing, WANG HuiFang, WANG YiQin

    (Laboratory of Syndromes of Traditional Chinese Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China; Central Laboratory, Shuguang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200021, China)

    ABSTRACT Objective: To explore the immunological mechanism of exfoliative tongue fur in children with asthma. Methods: Thirtynine children with asthma, twentyeight children with repetitive respiratory tract infection (nonasthma) and eleven healthy children were divided into five groups, which were asthma with exfoliative fur or with nonexfoliative fur groups, nonasthma with exfoliative fur or with nonexfoliative fur groups and normal control group. The concentrations of keratin 13 and bcl2 in cells exfoliated from tongue fur were detected by immunohistochemical method. The expression levels of blood cell chemokine receptor3 (CCR3) and CD4+ were examined by flow cytometry, and the levels of serum cortisol and IgE were detected by radioimmunoassay. Results: The levels of blood CD4+ and CCR3 of children with asthma and exfoliative fur were higher than those in the asthma with nonexfoliative fur group and the normal control group (P<0.05). The serum level of cortisol in the groups of asthma with exfoliative fur and nonasthma with exfoliative fur were lower than that in the other groups (P<0.05). The serum levels of IgE in asthma with exfoliative fur or with nonexfoliative fur groups were higher than that in the other groups (P<0.05). Concentrations of keratin 13 in the cells exfoliated from tongue fur in the groups of asthma or nonasthma with exfoliative fur were lower than that of the other groups (P<0.05). There was no significant difference of expression level of bcl2 in the cells exfoliated from tongue fur among these five groups. Conclusion: There is a reasonably close relationship between the formation of exfoliative tongue fur and the immune system such as low level of serum cortisol and high levels of blood CD4+ and CCR3, which may all promote the formation of exfoliative fur. The disability of keratinization and apoptosis of epithelial cells of tongue may also be one cause for its formation. ......

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