汉滩病毒_____汉滩病毒；糖蛋白；基因克隆

汉滩病毒西安分离株84FLi M片段的克隆

http://www.100md.com 《第四军医大学学报》 2006年第7期

汉滩病毒,,汉滩病毒；糖蛋白；基因克隆,汉滩病毒西安分离株84FLiM片段的克隆,0引言,1材料和方法,2结果,3讨论,【参考文献】

     Cloning of fulllength complementary DNA of M gene of 84FLi， a Hantaan virus isolated from Xian

    LI XinHong，ZHANG Yan，ZHANG Ye，WANG PingZhong，HUANG ChangXing，BAI XueFan

    Center of Infectious Diseases of Chinese PLA，Tangdu Hospital，Fourth Military Medical University，Xian 710038，China

    【Abstract】 AIM: To clone the fulllength complementary DNA of M gene of 84FLi，a Hantaan virus. METHODS：Total viral RNA was extracted and complementary DNA was synthesized by reverse transcription as template for PCR. Two PCR products-84M2.0(1-2058) and 84M1.6(1964-3616), containing coding region for G1 and G2，respectively，were inserted into pMD18T vector. Recombinant pMD84M2.0 and pMD84M1.6 were then transformed into competent E.coli. CDNA clone containing the fulllength complementary DNA of M gene was obtained by enzyme digestion and ligation. PCR and restriction analysis were performed to identify the positive clone. RESULTS：Two cDNA clones containing the coding region for G1 and G2 were constructed. Then cDNA clone of the fulllength M gene was obtained. CONCLUSION：The fulllength complementary DNA of M gene of 84FLi was cloned successfully. ......

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