甘氨酸对内毒素性肝损害保护作用的机制
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王 强, 张 涛, 陈叶恒, 余和斌, 游海波
甘氨酸;肝脏;内毒素;Toll样受体4王强,张涛,陈叶恒,余和斌,游海波.甘氨酸对内毒素性肝损害保护作用的机制. 世界华人消化杂志2006;14(16)1561-1565,甘氨酸对内毒素性
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王强, 张涛, 陈叶恒, 余和斌, 游海波, 重庆市长寿区人民医院肝胆外科 重庆市 401220
王强, 重庆市长寿区人民医院肝胆外科主任, 副主任医师, 主要从事肝胆外科基础与临床研究. 国家自然科学基金资助项目, No. 30471696
通讯作者: 游海波, 401220, 重庆市, 长寿区人民医院肝胆外科. youhaibo0427@sohu.com
电话: 023-40405623
收稿日期: 2006-03-18 接受日期: 2006-04-06
Protection mechanisms of glycine against liver injury induced by liopolysaccharide
Qiang Wang, Tao Zhang, Ye-Heng Chen, He-Bin Yu, Hai-Bo You
Qiang Wang, Tao Zhang, Ye-Heng Chen, He-Bin Yu, Hai-Bo You, Department of Hepatobiliary Surgery, People’s Hospital of Changshou District, Chongqing 401220, China
Supported by National Science Foundation of China, No. 30471696
Correspondence to: Hai-Bo You, Department of Hepatobiliary Surgery, People’s Hospital of Changshou District, Chongqing 401220, China. youhaibo0427@sohu.com
Received: 2006-03-18 Accepted: 2006-04-06
Abstract
AIM: To explore the protective mechanisms of glycine on liopolysaccharide (LPS)-induced liver injury.
METHODS: BABL/c mice were randomly divided into LPS group (n = 50), glycine group (n = 50), and control group (n = 50). The animals in LPS and glycine groups were intraperitoneally injected with 10 mg/kg LPS to establish the liver injury model, while animals in glycine group were pretreated with 50 g/L glycine-containing food 3 d before modeling. The mice in control group received the same amount of normal saline. Liver samples were collected to observe histopathological changes. The levels of tumor necrosis factor-a (TNF-a) and interleukin-10 (IL-10) in plasma were measured by enzyme linked immunosorbent assay (ELISA). The mRNA expression of TNF-a, IL-10 and toll like receptor 4 (TLR4) in hepatic tissues were detected by reverse transcription-polymerase chain reaction (RT-PCR). The expression of TLR4 protein in liver was detected with immunohistochemistry.
RESULTS: Glycine improved the survival rates of the mice, and attenuated LPS-induced pathological changes of the liver tissues significantly ......
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