Survivin反义寡核苷酸抑制肝癌移植瘤生长的作用
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高 鹏, 张刚庆, 颜 政, 方驰华
反义寡核苷酸;肝癌;Survivin;疾病模型;裸鼠高鹏,张刚庆,颜政,方驰华.Survivin反义寡核苷酸抑制肝癌移植瘤生长的作用. 世界华人消化杂志2006;14(16)1627-16
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高鹏, 广东省第二人民医院普外科 广东省广州市 510317
张刚庆, 中山大学附属第三医院、广东省第二人民医院普外科博士后科研基地 广东省广州市 510317
颜政, 方驰华, 南方医科大学附属珠江医院肝胆外科 广东省广州市 510282
通讯作者: 方驰华, 510282, 广东省广州市工业大道253号, 南方医科大学珠江医院肝胆外科. fch58520@sina.com
电话: 020-61643211 传真: 020-61643296
收稿日期: 2006-02-13 接受日期: 2006-03-07
Inhibitory effect of Survivin antisense oligonucleotide on growth of human hepatic carcinoma transplanted subcutaneously
Peng Gao, Gang-Qing Zhang, Zheng Yan, Chi-Hua Fang
Peng Gao, Department of General Surgery, Guangdong Second People’s Hospital, Guangzhou 510282, Guangdong Province, China
Gang-Qing Zhang, Department of General Surgery, the Second People’s Hospital of Guangdong Postdoctoral Workstation, the Third Hospital of Sun Yat-Sen University, Guangzhou 510317, Guangdong Province, China
Zheng Yan, Chi-Hua Fang, Department of Hepatobiliary Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, Guangdong Province, China
Correspondence to: Dr. Chi-Hua Fang, Department of Hepatobiliary Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, Guangdong Province, China. fch58520@sina.com
Received: 2006-02-13 Accepted: 2006-03-07
Abstract
AIM: To explore the effects of liposomes Survivin antisense oligonucleotides (ASODN) on the growth of human hepatic carcinoma transplanted subcutaneously in nude mice.
METHODS: Human hepatic carcinoma transplanted subcutaneously was established in nude mice. Then the animals were randomly divided into 6 groups: control group (A), liposome group (B), sense oligonucleotide (SODN) group (C), 200 (D), 400 (E) and 600 mg/L (F) ASODN group. After treatment with the corresponding methods for 2, 4, 8, 12, 16, 20 d, the expression of Survivin mRNA and protein detected by reverse transcription-polymerase chain reaction and Western blot, respectively.
RESULTS: The growth of the tumors was significantly inhibited in all ASODN groups as compared with that in the control, liposome and SODN group after treatment for 20 d ......
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