幼鼠肠缺血/再灌注损伤肠组织TNF-α和c-fos mRNA的表达
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李春艳, 张 莹, 崔泽实, 卢 瑶, 于 明
肠;缺血,再灌注;肿瘤坏死因子;c-fos基因李春艳,张莹,崔泽实,卢瑶,于明.幼鼠肠缺血,再灌注损伤肠组织TNF-a和c-fosmRNA的表达. 世界华人消化杂志2006;14(16)1
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李春艳, 张莹, 崔泽实, 卢瑶, 中国医科大学实验技术中心三部 辽宁省沈阳市 110001
于明, 中国医科大学第二临床医院小儿外科 辽宁省沈阳市 110001
通讯作者: 李春艳, 110001, 辽宁省沈阳市, 中国医科大学实验技术中心三部. lcy_cn@hotmail.com
电话: 024-23256666-5100 传真: 024-23261099
收稿日期: 2006-03-24 接受日期: 2006-04-16
Expression of tumor necrosis factor-α and c-fos mRNA in immature rats’ model of gut ischemia/reperfusion injury
Chun-Yan Li, Ying Zhang, Ze-Shi Cui, Yao Lu, Ming Yu
Chun-Yan Li, Ying Zhang, Ze-Shi Cui, Yao Lu, the Third Division of Medical Laboratory Technology Center, China Medical University, Shenyang 110001, Liaoning Province, China
Ming Yu, Department of Pediatric Surgery, the Second Affiliated Hospital of China Medical University, Shenyang 110001, Liaoning Province, China
Correspondence to: Dr. Chun-Yan Li, the Third Division of Medical Laboratory Technology Center, China Medical University, Shenyang 110001, Liaoning Province, China. lcy_cn@hotmail.com
Received: 2006-03-24 Accepted: 2006-04-16
Abstract
AIM: To analyze the expression patterns of tumor necrosis factor-a (TNF-a) and c-fos mRNA in immature rats after gut ischemia/reperfusion (I/R) injury.
METHODS:The left laparotomy was performed and the superior mesenteric artery (SMA) was occluded by artery clamp to establish the model of gut I/R injury. The expression of TNF-a and c-fos mRNA were detected by reverse transcription polymerase chain reaction (RT-PCR).
RESULTS:In comparison with sham operation group, an increase in TNF-a mRNA was detected after 30-min ischemia (1 ......
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