HPLC法测定库拉索芦荟中芦荟苷的含量
芦荟苷,,芦荟苷;色谱法,高压液相;,库拉索芦荟,0引言,1材料和方法,2结果,3讨论,【参考文献】
Determination of aloin in aloe barbadensis by HPLCCHEN Hui, JIANG XiuMei, WANG QiaoFeng ,ZHOU Jun
Department of Chemistry, School of Pharmacy, Fourth Military Medical University,Xian 710033,China
【Abstract】 AIM: To describe a simple and rapid reversed-phase high performance liquid chromatography method in applied to the content of aloin in aloe barbadensis miller. METHODS: A column ZODBAXC18(46 mm×150 mm,5 μm)was used with a mobile phase of methanolwaterphosphate buffer(45∶55∶05), the flow rate being 1 mL/min, detection wavelength at 356 nm and the column temperature being 25℃. RESULTS: The linear the range of aloin was between 0596119mg with a correlation coefficient of 09996 and the average recovery of the method was 978% and RSD is 102%. CONCLUSION: As the method is convenient, economical and accurate with good reproducibility, it can be used in the quantitative analysis of aloin in aloe barbadensis.
【Keywords】 aloin;chromatography,high pressure liquid;aloe barbadensis
【摘要】 目的: 建立用反相高效液相色谱测定库拉索芦荟中芦荟苷含量的方法.方法: 采用高效液相色谱法 ......
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