肝癌_____]_肝癌;_肿瘤转移;

大鼠移植性肝癌模型生物学行为与分期的初步探讨

http://www.100md.com 《中西医结合学报》 2005年第2期

肝癌,,],肝癌;,肿瘤转移;,大鼠;,实验动物模型;,分期,1材料与方法,2结果,3讨论,[参考文献]

     [摘要] 目的：探讨可能反映人类原发性肝癌发生发展的大鼠移植性肝癌模型的分期标准。方法：采用完整瘤组织块肝内种植法复制大鼠移植性肝癌模型，在不同时期分别观察模型大鼠肝癌体积和体质量等一般状况，用全自动生化仪检测大鼠血清白蛋白含量，病理切片观察肝癌组织及邻近脏器侵润、转移情况。结果：模型大鼠体质量减轻及腹水生成平均出现在造模后第12、18天；第11天白蛋白含量为(35.32±2.45) g/L，与造模前比较无统计学差异(P>0.05),第18天白蛋白含量为(26.00±3.37) g/L，与第11天比较差异有统计学意义(P<0.05)；模型第23天出现不同程度的脏器转移，其中血性腹水发生率为56.25%。结论：大鼠移植性肝癌模型第5～11天相当于人类早期肝癌。模型第18天后相当于临床晚期肝癌。该分期标准可能用于指导原发性肝癌实验性治疗策略的选择。

    [关键词] 肝癌; 肿瘤转移; 大鼠; 实验动物模型; 分期

    Preliminary study on biological behavior and staging of transplanted hepatoma in rats

    GU Wei, SHEN Jie, ZHAI XiaoFeng

    (Department of Traditional Chinese Medicine, Changhai Hospital, Second Military Medical University, Shanghai 200433, China)

    ABSTRACT Objective: To establish a staging standard for a transplanted hepatoma model in rats, which may be equivalent to the human primary liver cancer. Methods: A transplanted hepatoma model was made in SD rats by implantation of histologically intact Walker256 tumor fragment into the left lateral lobe of the liver. The body weight, tumor volume and serum albumin level of the rats were measured and assayed in different periods. Pathological sections were also fabricated to detect the invasion and metastasis of the neighboring tissues. Results: The body weight loss and ascites occurred at the 12th and 18th day separately. The serum albumin level was (35.32±2.45) g/L at the 11th day, which showed no statistical difference as compared with the healthy rats (P>0.05), and the albumin level at the 18th day was significantly lower than that at the 11th day (P<0.05). At the 23rd day, 56.25% of the rats developed bloody ascites. Conclusion: The period between 511 days in the model rats may be correspondent to the early stage of human primary liver cancer, and the biological behavior of the model rats at the18th day may be correspondent to the late stage of human primary liver cancer. This staging standard might be the guideline for model selection in experimental study of primary liver cancer. ......

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