【摘要】 目的 研究雌黄(As2S3)能否诱导急性粒细胞白血病细胞株(HL-60)凋亡与坏死。方法 以不同浓度的As2S3在不同的时间处理体外培养的HL-60细胞株，应用细胞生长测定、形态学观察及流式细胞仪检测等多种方法，在体外研究As2S3对HL-60细胞凋亡和增殖抑制的作用。通过对细胞进行Annexin V和碘化丙锭(PI)双重染色，应用流式细胞仪可以区分活细胞、凋亡细胞与坏死细胞。结果 2.5μmol/L As2S3作用18h后诱导HL-60小部分细胞凋亡和大部分细胞坏死,7.5μmol/L As2S3作用8h后可引起HL-60细胞凋亡。结论 As2S3可诱导HL-60细胞凋亡与坏死，有必要进一步探索其对急性粒细胞白血病治疗的价值。

    【关键词】 雌黄(三硫化二砷)；HL-60细胞；凋亡

    Experimental study on As2S3 (Orpiment) induced apoptosis and necrosis of human leukemia cell line HL-60

    HE Zhi-jie.Department of Hematology and Chemotherapy, Suzhou Municipal Hospital，Suzhou 215002,P.R.China

    【Abstract】 Objective The current study was designed to investigate whether As2S3(Orpiment) will induce apoptosis and necrosis of human acute myloid leukemia cell line HL-60. Methods Acute leukemia cell line HL-60 was used as in vitro model. HL-60 cell line was treated with As2S3 at different concentrations and different period, and cell growth was analyzed with trypan blue exclusion. Apoptosis was assessed with cell morphology (including microscope and electron microscope)and flow cytometry, the latter can distinguish live cell, apoptotic cell and necrotic cell through double staining of Annexin V and propidium iodide, of which fluorescent intensities were measured on flow cytometry.Results 2.5μmol/L of As2S3 could induce most of cell HL-60 necrosis and a small part of cell apoptosis within 18h ......