SFE technology of indirubin from Daqingye and content determination of indirubin

    LI Hui, WANG Siª²Wang, WANG Jianª²Bo

    Institute of Pharmacology, Department of Pharmacy, Fourth Military Medical University, Xi¬ðan 710033, China

    ¡¾Abstract¡¿ AIM: To use HPLC for the content determination of indirubin in supercritical fluid extraction (SFE) material from Daqingye and to optimize the process of SFE. METHODS: Content of indirubin was used as index to evaluate the technologies based on orthogonal design, in which 4 factors considered were extraction temperature and pressure, separation temperature and pressure. A HPLC system was used to determine the content of indirubin. RESULTS: Optimal program was as follows: extraction temperature 50¡æ and pressure 40 MPa, separation temperature 40¡æ and pressure 8 MPa. The average recovery of indirubin was 102.8% and RSD was 1.48%. CONCLUSION: The extraction temperature, extraction pressure and separation pressure all have significant effects on the extraction rate of indirubin. The HPLC method is simple, precise and suitable for the content determination of indirubin in SFE material of Daqingye. ......