Adenovirusª²mediated herpes simplex virus thymidine kinase gene transfer under the driving of KDR promoter in treatment of experimental human nasopharyngeal carcinoma in nude mice

    ZHU Qianª²Yuan, LI Baoª²Jin, GUO Jian

    Department of Otorhinolaryngology, Affiliated Hospital Medical College, Jinggangshan University, Ji¬ðan 343000, China, Shenzhen Hospital, Peking University, Shenzhen 518036, China

    ¡¾Abstract¡¿ AIM: To investigate the therapeutic efficacy of adenovirusª²mediated herpes simplex virus thymidine kinase (HSVª²tk) gene transfer under the driving of KDR promoter (AdKDRª²tk) in combination of ganciclovir(GCV) against human nasopharyngeal carcinoma(NPC) in nude mice. METHODS: CNEª²2 cell line was implanted subcutaneously into 36 nude mice, which were subsequently divided into 3 groups (n=12 each group): AdKDRª²tk/GCV group, AdCMVª²tk/GCV group (under the driving of CMV promoter) and saline/GCV group. Then the selective intratumoral injection of recombinant adenovirus or saline was performed in all nude mice ......