RegⅣ基因__(期刊论文)

RegⅣ基因在结直肠癌细胞系中的mRNA表达及pcDNARegⅣ的构建与转染

http://www.100md.com 《东南大学学报(医学版)》 2006年第3期

RegⅣ基因,,]RegⅣ基因；结直肠癌；重组质粒；基因表达；基因转染,[摘要],[关键词],1材料与方法,2结果

     [摘要]

    目的：探讨RegⅣ基因在4种结直肠癌细胞系中的表达水平，构建并转染pcDNARegⅣ重组质粒，为阐明RegⅣ基因在结直肠癌发生发展中的作用机制奠定基础。方法：采用RTPCR方法检测HT29、Caco2、Sw480、LoVo细胞系的RegⅣ基因表达水平，将获得目的基因RegⅣ克隆于pcDNA3.1/(-)质粒上，用PCR、酶切进行鉴定后，脂质体转染至低表达甚至不表达RegⅣ的结直癌细胞系，用G418筛选后进行RTPCR鉴定。结果：HT29、Caco2细胞系RegⅣ基因高表达，Sw480细胞系表达较前两者弱，LoVo细胞系RegⅣ阴性表达；构建的重组质粒中含有RegⅣ基因，经pcDNARegⅣ转染的LoVo细胞RegⅣ基因呈阳性表达。结论：RegⅣ基因在不同的结直肠癌细胞系中表达水平不同。成功构建和转染了pcDNARegⅣ,可使得RegⅣ(-)的LoVo细胞系RegⅣ基因呈阳性表达。

     [关键词]RegⅣ基因；结直肠癌；重组质粒；基因表达；基因转染

    [中图分类号]Q786；Q785

    Expression of RegⅣ mRNA in colorectal cancer cells and the transfection of constructed recombinant plasmid with pcDNARegⅣ

    GAO Feng，ZHENG Shengqin，ZHANG Yuwei, JIN Yueling, TIAN Xiaoqiang，HUANG Peilin

    (Department of Pathology and Pathophysiology, School of Basic Medical Science, Southeast University，Nanjing 210009，China)

    Abstract：Objective To investigate the expression of RegⅣ in four colorectal cancer cells and construct recombinant plasmid pcDNARegⅣ and transfect it for further study of RegⅣ in colorectal cancer cells.Methods RegⅣ obtained from colorectal cancer cells by RTPCR was cloned into pcDNA3.1/(-) to form pcDNARegⅣ which was transfected into colorectal cancer cell with less or no expression of RegⅣ via liposome after identification of PCR ......

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