[摘要]目的 观察康寿灵含药血清对过氧化氢(H2O2 )诱导的SH-SY5Y神经细胞损伤的保护作用。方法采用体外细胞培养的方法，建立SH-SY5Y神经细胞H2O2损伤模型。通过观察细胞形态，测定细胞存活率(MTT 法)、乳酸脱氢酶(LDH)活性，检测康寿灵含药血清对SH-SY5Y神经细胞损伤的保护作用。结果 同模型组比较，体积分数为0.05、0.10、0.15的康寿灵含药血清能减轻H2O2引起的SH-SY5Y神经细胞的损伤，明显提高细胞的存活率，减少LDH的释放量。结论 康寿灵含药血清对H2O2 诱导的SH-SY5Y神经细胞损伤具有明显的保护作用。

    [关键词]康寿灵;过氧化氢;SH-SY5Y神经细胞;药物作用

    PROTECTIVE EFFECT OF KANGSHOULING-CONTAINING SERUM ON DAMAGE OF CULTURED SH-SY5Y CELLS IN VITRO INTRODUCED BY HYDROGEN PEROXIDE

    LIU HONG-LING， SUN XIANG-HONG， JIANG HUI-QING， et al

    (Depart-ment of Pharmacy，The Affiliated Hospital of Qingdao University Medical College， Qingdao 266003，China)

    [ABSTRACT] Objective To investigate the protective effect of Kangshouling-containing serum on the damage of cultured SH-SY5Y cells. Methods The damage of cultured SH-SY5Y cells was made by hydrogen peroxide. The protective effect of Kangshouling-containing serum was observed by morphological identification， colorimetric MTT assay， and LDH activity. Re-sults Kangshouling-containing serum (0.05，0.10，0.15 ) can relieve the damage of SH-SY5Y cells and increase the survival of the cells ......