[摘要]目的 探讨人脐带间质干细胞(MSC)的分离、纯化、扩增方法，研究其基本生物学特性。方法 无菌条件下收集剖宫产新生儿脐带，分别用组织块贴壁法和酶消化法获取脐带间质干细胞，进行原代培养。应用DMEM/F12培养液进行纯化和扩增培养。用流式细胞仪检测MSC的细胞表面标志。绘制细胞生长曲线并测定细胞周期。结果 两种分离方法均可获得MSC，原代培养12～14 d后可达90%融合，细胞可传代20代以上。流式细胞仪检测结果显示，脐带MSC强表达CD13、CD29、CD44、CD105，弱表达CD106，不表达CD34、CD11a、CD14、CD31、CD45。结论 脐带MSC在体外有较强的增殖能力，可作为组织工程的种子细胞。

     [关键词] 脐带;间质干细胞;生物学;细胞分离

    BIOLOGICAL STUDY OF HUMAN UMBILICAL CORD DERIVED MESENCHYMAL STEM CELLS

    YUAN YUAN， WANG YUAN-LI， YANG SHU-YUAN， et al

    (Department of Neurosurgery， General Hospital of Tianjin Medical University，Tianjin 300052， China)

    [ABSTRACT]ObjectiveTo explore the isolation， purification and expansion method of human umbilical cord derived mes-enchymal stem cells (UCMSC). MethodsThe umbilical cords from cesarean newborns were collected in sterile condition. Me- senchymal stem cells were obtained by enzyme digest method and cultured in DMEM/F12 medium. Surface antigens of UCMSC were detected by FACS. The cell growth curve and cell cycle of UCMSC were analyzed. ResultsMSCs could be obtained by both of the two methods. After 12-14 days of primary culture ......