聚乳酸-O-羧甲基壳聚糖纳米粒子的制备及其对培养猪肝细胞的影响
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陈 钟, 戴新征, 杨欣荣, 祝文彩
聚乳酸-O-羧甲基壳聚糖;纳米材料;肝细胞;培养;肝细胞移植,陈钟,戴新征,杨欣荣,祝文彩,陈钟,通讯作者,Preparationofp
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陈钟, 戴新征, 杨欣荣, 祝文彩, 南通大学附属医院肝胆外科, 南通大学肝胆外科研究所 江苏省南通市 226001
陈钟, 2002年南京大学博士后, 教授, 主任医师, 主要从事肝细胞移植和肝胆外科研究.
江苏省卫生厅重大基金资助项目, No. H200215
南通大学创新基金资助项目, No. CX0301
通讯作者: 陈钟, 226001, 江苏省南通市西寺路20号, 南通大学附属医院肝胆外科. chenz@ahnmc.com
电话: 0513-85829806 传真: 0513-85106369
收稿日期: 2006-04-07 接受日期: 2006-05-08
Preparation of polylactic acid-O-carboxymethylated chitosan nanoparticles and their influences on the cultured porcine hepatocytes
Zhong Chen, Xin-Zheng Dai, Xin-Rong Yang, Wen-Cai Zhu
Zhong Chen, Xin-Zheng Dai, Xin-Rong Yang, Wen-Cai Zhu, Department of Hepatobiliary Surgery, the Affiliated Hospital of Nantong University; Institute of Hepatobiliary Surgery, Nantong University, Nantong 226001, Jiangsu Province, China
Supported by the Key Project Foundation from the Health Department of Jiangsu Province, No. H200215, and the Innovation Foundation of Nantong University, No. CX0301
Correspondence to: Zhong Chen, Department of Hepatobiliary Surgery, the Affiliated Hospital of Nantong University, 20 Xisi Road, Nantong 226001, Jiangsu Province, China. chenz@ahnmc.com
Received: 2006-04-07 Accepted: 2006-05-08
Abstract
AIM: To prepare polylactic acid-O-carboxyme-thylated chitosan (PLA-O-CMC) nanoparticles and evaluate their influences on the morphology and functions of cultured porcine hepatocytes.
METHODS: PLA-O-CMC nanoparticles were prepared from PLA and O-CMC by ultrasonic method. The nanoparticles were characterized by atomic force microscopy and X-ray photoelectron spectrometry. Isolated porcine hepatocytes were divided into two groups: commonly culturing group and nanomaterial group (co-culturing hepatocytes with PLA-O-CMC nanoparticles). The morphology and functions of the cultured hepatocytes were observed within 1 wk.
RESULTS: PLA-O-CMC nanoparticles were successfully prepared, and the diameter was 300-500 nm under atomic force microscope ......
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