摘 要 目的：建立测定复方甲硝唑片中甲硝唑与维生素B6含量及溶出度的方法。方法：高效液相色谱法。选用钻石C18柱，流动相：ψ(甲醇∶0.075 mol/L磷酸二氢钾溶液)=13∶87，检测波长：291 nm。结果：同时测定了甲硝唑与维生素B6的含量。甲硝唑平均回收率为99.7﹪，RSD为0.17﹪，n=6；维生素B6平均回收率为100.1﹪，RSD为0.27﹪，n=6。线性范围甲硝唑为20.12～100.60 μg/mL、维生素B6为2.04～10.20 μg/mL。结论：本法简便、快速、准确，可用于复方甲硝唑片的质量控制。

    关键词 HPLC；甲硝唑；维生素B6；溶出度

    Study on the determination and dissolubility of metronidazole

    compound tablets by HPLC

    Abstract Objective: To establish a HPLC method to determine the content of metronidazole and vitamin B6 and the dissolubility of metronidazole compound tablets. Method: The HPLC system consisted of Diamonsil C18 column, and a mixture of methanol-0.075 mol/L KH2PO4(13∶87) was used as mobile phase. The detection wavelength was set at 291 nm. Results: The contents of metronidazole and vitamin B6 were determined respectively. The average recovery for metronidazole was 99.7﹪ ......