骨髓间质干细胞_

定向诱导兔骨髓间质干细胞分化为神经元样细胞的研究

http://www.100md.com 《广东药学院学报》 2001年第4期

骨髓间质干细胞,,骨髓间质干细胞；神经元；分化；硫代甘油,1材料和试剂,2方法,3结果,4讨论,参考文献

     摘要目的：体外定向诱导兔骨髓间质干细胞(MSC)分化为神经元样细胞。方法：采用FicollPaque液(1.083 g/L)离心分离兔MSC，体外扩增，硫代甘油等试剂的无血清达乐伯克改良必需基本培养基(DMEM)诱导MSC分化为神经元。免疫组化鉴定神经元烯醇化酶(NSE)、神经丝蛋白(NF)、胶质纤维酸性蛋白(GFAP)的表达。结果：兔骨髓间质干细胞在体外扩增原代可获得5×105、10代可获得2×1010个细胞。加入硫代甘油诱导后，MSC胞体收缩，突起伸出；免疫组化显示诱导出的神经元样细胞NSE、NF、表达阳性，GFAP阴性。结论：兔骨髓间质干细胞在体外可以分化为神经元样细胞。

    关键词骨髓间质干细胞；神经元；分化；硫代甘油

    Induction of the differentiation of rabbits mesenchymal stem cells into neuronlike cells with monothioglycerol

    ZHANG Lirong1，XIA Wenjie2,LI Shu nong2

    (1.Department of Pathophysiology,Guangdong College of Pharmacy,Guangzhou,Guangdong 510224; 2.Department of Pathophysiology,Sun Yatsen University of Medical Sciences)

    Abstract Objective:To induce the differentiation of rabbit bone marrow mesenchymal stem cells (rMSC) into neuronlike cells in vitro. Methods: rMSCs were separated from rib marrow with FicollPaque reagent and expanded in culture medium. Differentiation of rMSCs into neurons were induced with DMEM/monothioglycerol. Neuronspecific enolase(NSE),neurofilament (NF),glial fibrillary acidic protein (GFAP) were detected by immunohistochemistry. Results:rMSCs were expanded in culture for 10 passages.After the induction by adding monothioglycerol the MSCs displayed a fibroblastlike morphology. Immunohistochemical assay showed that there were positive expressions of NSE and NFM,on the neuronlike cells,without the expression of the glial astrocyte marker GFAP.Conclusion:It suggests that the differentiation of rMSC into neurons can be induced in vitro. ......

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