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HPLC法测定莲芝消炎胶囊中穿心莲内酯的含量
http://www.100md.com 《广东药学院学报》 2002年第2期
莲芝消炎胶囊,,莲芝消炎胶囊;穿心莲内酯;,HPLC,1实验部分,2方法与结果,3结论,参考文献
     摘 要 建立了测定莲芝消炎胶囊中穿心莲内酯含量的HPLC法。色谱条件为:Diamonsil-C18柱,ψ(甲醇∶水)=60∶40为流动相,流速0.8 mL/min,检测波长225 nm,柱温为室温。穿心莲内酯在43.6~261.6μg/mL浓度范围内与峰面积呈良好的线性关系(r=0.9998),平均回收率98.1%,RSD=1.98%。

    关键词 莲芝消炎胶囊;穿心莲内酯; HPLC

    Determination of the andrographolide in Lianzhixiaoyan capsules by RPHPLC

    Abstract A RPHPLC method for the determination of andrographolide in Lianzhixiaoyan capsules was established. A Diamonsil C18 column was used,with the mobile phase of methanolwater(60∶40). The flow rate was 0.8 mL/min, and the detection wavelength was 225 nm. The concentration of andrographolide was linearly related to its peak area in the range of 43.6~261.6μg/mL ......

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