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编号:11013605
高效液相色谱法考察复方头孢氨苄胶囊的溶出度
http://www.100md.com 《广东药学院学报》 2001年第2期
头孢氨苄,,高效液相色谱法;复方头孢氨苄胶囊;头孢氨苄;甲氧苄啶;溶出度,1仪器与试药,2色谱条件,3实验方法及结果,4讨论,参考文献
     摘 要 采用反相高效液相色谱外标法,对复方头孢氨苄胶囊溶出度进行考察。 方法:采用转蓝法,以4%乙酸溶液为溶剂,转速为100 r/min,时间为45 min。色谱条件:C18反相色谱柱为固定相,V(水)∶V(甲醇)∶V(3.86%乙酸钠溶液)∶V(4%乙酸溶液)=742:240:15:3为流动相,流速为1.2 mL/min,紫外检测波长为254 nm,在此条件下头孢氨苄和甲氧苄啶两组分能很好地分离。本法的线性范围分别是头孢氨苄为:27.6~276 μg/mL,甲氧苄啶为:5.56~55.6 μg/mL,平均回收率为头孢氨苄:99.32%,RSD=0.78%;甲氧苄啶:100.1%, RSD=0.57%。

    关键词 高效液相色谱法;复方头孢氨苄胶囊;头孢氨苄;甲氧苄啶;溶出度

    Determination of dissolution of compound cephalexin capsule by HPLC

    MAO Qiulan(Foshan Kang Bao Sun Pharmacy Co. Ltd. , Foshan,Guangdong 528000)

    Abstract A reversedphase HPLC (RPHPLC) method for the determination of the dissolution of compound cephalexin capsule was developed. A basket stirring technique was applied, with 4% acetic acid solution as dissolvent, at speed of 100 r/min for 45 min . A C18 reversedphase column was used as fixed phase and watermethanol3.86% sodium acetate40% acetic acid (742∶240∶15∶3) as mobile phase. The flow rate was 1.2 ml/min and UV detection was performed at 254 nm. Cephalexin and Trimethoprim (TMP) could be separated in this condition. The linear ranges were 27.6~276 μg/mL for Cephalexin and 5.56~55.6 μg/mL for TMP. The recovery and RSD were 99.32% and 0.78% for Cephalaxin and 100.1% and 0.57% for TMP respectively. ......

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