纳升电喷雾串联质谱_

纳升电喷雾串联质谱鉴定重组人甲状旁腺素(134)

http://www.100md.com 《分析化学》 2006年第5期

纳升电喷雾串联质谱,,重组人甲状旁腺素(134)，纳升电喷雾串联质谱，肽序列分析,摘要,关键词,1引言,2实验部分,3结果与讨论,Refer

     摘要

    用先进的纳升电喷雾四极杆飞行时间串联质谱鉴定重组人PTH(134)。通过ESIMS测定重组人PTH(134)分子量及MS/MS对其胰蛋白酶酶解后的肽段的序列和数据库查询进行结构鉴定。重组PTH(134)测定分子量为4115.21，与理论值相比测定相差0.06%。MS/MS测定出其中双电荷离子峰m/z 728.4 肽段序列为VSEIQLMHNLG，以及其他3个单电荷离子峰的序列。数据库检索后确定重组表达的PTH(134)一级结构完全正确，纳升电喷雾串联质谱以其灵敏、快速和准确为蛋白质鉴定提供了有效的手段。

     关键词 重组人甲状旁腺素(134)，纳升电喷雾串联质谱，肽序列分析

    Identification of Recombinant Human Parathyroid Hormone (134) by Nanoelectrospray IonizationQuadrupoleTime of Filght Tandem Mass Spectrometry

    Chen Jing， Sun Hongying， Chen Shuqing*

    (Department of Biochemistry Pharmacy, College of Pharmaceutical Sciences,Zhejiang University, Hangzhou 310031)

    Abstract Recombinant human parathyroid hormone (PTH) (134) was identified by new technique nanoelectrospray ionization (ESI)quadrupoletime of flight (TOF) tandem mass spectrometry. The measured molecular weight of PTH(134) was 4115.21 by ESImass spectrometry (MS) and measurement percent error was only 0.06 %. Digested PTH(134) by trypsin at 37℃ over night. The tryptic digested peptides were measured and then four charged peptides were selected to determine amino acid sequence by MS/MS. Then their sequences were interpreted from a ......

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