自体DC体外增强结肠腺癌患者TDLN细胞的抗肿瘤活性
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彭 正, 陈 凛, 李 荣, 李 力, 董艳芬,
肿瘤引流区淋巴结细胞;树突状细胞;抗肿瘤活性;结肠腺癌彭正,陈凛,李荣,李力,董艳芬,张磊.自体DC体外增强结肠腺癌患者TDLN细胞的抗肿瘤活性. 世界华人消化杂志2006;14(18)1
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彭正, 陈凛, 李荣, 董艳芬, 解放军总医院普通外科 北京市 100853
李力, 张磊, 解放军总医院普通外科研究所 北京市 100853彭正, 1994年第四军医大学学士, 2003年解放军总医院硕士, 主治医师.
通讯作者: 彭正, 100853, 北京市, 解放军总医院普通外科. pengzham@yahoo.com.cn
电话: 010-66875143
收稿日期: 2006-05-19 接受日期: 2006-06-14
Autologous dendritic cells enhance anti-tumor activity of tumor-draining lymph node cells from colonic adenocarcinoma patients in vitro
Zheng Peng, Lin Chen, Rong Li, Li Li, Yan-Fen Dong, Lei Zhang
Zheng Peng, Lin Chen, Rong Li, Yan-Fen Dong, Department of General Surgery, General Hospital of Chinese PLA, Beijing 100853, China
Li Li, Lei Zhang, Institute of General Surgery, General Hospital of Chinese PLA, Beijing 100853, China
Correspondence to: Zheng Peng, Department of General Surgery, General Hospital of Chinese PLA, Beijing 100853, China. pengzham@yahoo.com.cn
Received: 2006-05-19 AccePted: 2006-06-14
AbstractAIM:To explore the enhancement of the anti-tumor activity of tumor-draining lymph node (TDLN) cells induced by autologous dendritic cells (DCs) in vitro.
METHODS:Peripheral blood mononuclear cells (PBMCs) were separated from the patients with colonic adenocarcinoma and then induced with granulocyte-macrophage colony-stimulating factor (GM-CSF), interleukin-4 (IL-4) and TNF-a for maturation. Thereafter, the autologous tumor lysate was used to sensitize DCs. TDLN cells were isolated from regional lymph nodes of the patients, purified, and incubated with interleukin-2 (IL-2). Finally, the TDLN cells were activated by the sensitized DCs or autologous tumor lysate, respectively, and the TDLN cells without activation served as controls. The cytotoxic activity of the activated TDLN cells to colonic adenocarcinoma cell line LS174T and human melanoma cell line A375 were comparatively analyzed ......
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