养阴抗毒胶囊对阴虚大鼠外周血T细胞亚群及IL1β,TNFα的影响
养阴抗毒胶囊;糖皮质激素;阴虚证;T细胞亚群;细胞因子;大鼠,,养阴抗毒胶囊;糖皮质激素;阴虚证;T细胞亚群;细胞因子;大鼠,养阴抗毒胶囊对阴虚大鼠外周血T细胞亚群及IL1β,TNFα的影响
Effect of Nourishing Yin Antitoxic Capsule on T lymphocyte subsets, IL1β and TNFα of peripheral blood in Yin deficiency rats induced by hydrocortisoneHOU Ying1, SHI HengJun1, CHEN Ru1, JU Yan1, CAO YunXin2
1Department of Traditional Chinese Medicine, Tangdu Hospital, Fourth Military Medical University, Xian 710038, China, 2Department of Immunology, School of Basic Medicine, Fourth Military Medical University, Xian 710033, China
【Abstract】 AIM: To explore the effect of Nourishing Yin Antitoxic Capsule (YC) on T lymphocyte subsets and IL1β, TNFα of peripheral blood in Yin deficiency rats induced by hydrocortisone. METHODS: Thirtytwo SD rats were randomly allocated to 4 groups: normal control group, Yin deficiency model control (YD) group, YC group and Liuwei Dihuang Balls (LD) group (with the action of nourishing Yin and replenishing kidney). Radioimmunity method was used to observe the contents of corticosteroid (CORT), IL1β and TNFα in blood serum at 9th day after medicine pretreatment and Yin deficiency model establishment. T lymphocyte subsets of peripheral blood in rats were detected by flow cytometer. RESULTS: Compared with the normal group, the contents of CORT were higher in YD group (P<0.05); and that could be reduced by YC and LD (P<0.05). Meanwhile the contents of IL1β and TNFα were increased significantly in YD group (P<0.05 and P<0.01); and they could be reduced by YC and LD (P<0.05) but ones of YC group approached more to normal control group. The percentages of CD4+ and CD8+ in YD group were increased (P<0.05), the ratio of CD4+/CD8+ was not different significantly between all groups. CONCLUSION: T lymphocyte subsets of peripheral blood are not significantly decreased in Yin deficiency syndrome induced by hydrocortisone. There are correlations between the increased contents of IL1β and TNFα and Yin deficiency syndrome induced by hydrocortisone. YC can decrease the contents of IL1β and TNFα, which might be one of the mechanisms of treating the adverse reaction of glucocorticoids. ......
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