摘要:目的 建立反相高效液相色谱-二极管阵列检测法同时测定食品中维生素A和维生素E(α-生育酚)的方法。 方法 取一定量食品样品加入无水乙醇、氢氧化钾及焦性没食子酸，80℃水浴皂化30min，石油醚提取后，氮气吹扫浓缩致干后用流动相溶解，进样5μl分析;色谱条件:色谱柱Supelco C 18 柱(10cm×2.1mm，5μm)，流动相甲醇:水(93+7)，流速0.4ml/min，柱温40℃，DAD扫描波长范围210～370nm，检测波长325nm(Vitamin A)和294nm(Vitamin E)。 结果 维生素A在1.0～100.0μg/L浓度范围内线性良好，相关系数r=0.9998，维生素E在0.10～10.0mg/L浓度范围内相关系数r=0.9991。维生素A和维生素E的平均回收率在96%以上。 结论 该法快速、灵敏、准确，样品处理简便易行，可用于食品中脂溶性维生素A和E的同时测定。

    关键词:维生素A;维生素E;高效液相色谱法

    Simultaneous detection of retinal andα-tocopherol from foods by using high performance liquid phase chromatogra-phy.

    WANG Hui，LIU Hong-he，YIN Jiang-wei.

    (Guanlan District for Health Prevention，Shenzhen518100，Guangdong，P.R.China)

    Abstract:Objective To determinate the concentrations of retinal andα-tocopherol in foods. Methods Food samples were added in anhydrous alcohol，potassium hydroxide and pyro-gallic acid.The mixture was shaken and saponified at80℃for30min-utes.The lipo-soluble vitamins were extracted by petroleum ether.The organic layer was dried by gas of N 2 ......