肝炎病毒_乙型；基因重组；真核表达_

重组乙型肝炎病毒P22e基因在HepG2细胞中的表达

http://www.100md.com 《第四军医大学学报》 2006年第11期

肝炎病毒,乙型；基因重组；真核表达,,肝炎病毒，乙型；基因重组；真核表达,【摘要】,【关键词】,0引言,1材料和方法,2结果,3讨论,【参考

     Construction of recombinant vector of HBVP22e gene and its expression in HepG2 cells

    DIAO ZhiHong, ZHANG MingXia, ZHU YouFu, HE HaiTang, WANG ZhanHui, CHEN JinJun, HOU JinLin

    Department of Infectious Diseases, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China

    【Abstract】 AIM: To construct the recombinant expression vector of pCDNA3.1+HBVP22e and transfect HepG2 cells for the expression of HBVP22e, and to assess its relations to HB. METHODS： HBVP22e cDNA was obtained from plasmid p3.8Ⅱ which carried 1.2 copies HBV (adr subtype) genome by PCR and inserted into universal vector pMD18T for identification of its DNA sequence. The recombinant plasmids were constructed by pCDNA3.1+ and transfected into HepG2 cells via liposom. HBVP22e protein was analyzed by immunohistochemistry and microparticle enzyme immunoassay. RESULTS： Expression vectors of recombinant pCDNA3.1+HBV P22e were successfully constructed and transferred into HepG2 cells. Its expressed steadily in HepG2 cells after cultured over several passages. CONCLUSION： HBVP22e can be expressed in HepG2 cells. ......

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