【摘要】 目的 利用针对人端粒酶RNA(hTR)的反义寡核苷酸(ASODN)和正义寡核苷酸(NODN)作用于人肝癌细胞SMMC-7721，比较ASODN和NODN作用后细胞培养液蛋白的变化。 方法 利用SELDI技术检测差异蛋白的表达变化。对照组为未加任何处理因素的SMMC-7721细胞。 结果 SELDI技术检测发现，ASODN作用组有40个差异蛋白分子低表达，3个差异蛋白分子高表达。NODN作用组有37个差异蛋白分子低表达，6个差异蛋白分子高表达。所有差异蛋白分子量均小于10000Da。ASODN作用组中有3个低表达蛋白在NODN作用组高表达，分子量分别为3011.99Da、3048.28Da和3248.75Da。 结论 ASODN和NODN作用SMMC-7721细胞后细胞培养液中所表达的差异蛋白十分相似。

    【关键词】 寡核苷酸类，反义;肝肿瘤

    Detection of differential protein in culture fluid of hepatocellular carcinoma cells treated by antisense oligodeoxynucleotide using SELDI

    WEI Xiao ，HE Min ，HUANG Yuan-jiao ，ZHU Miao ，RONG Min-hua ，QIN Jian ，ZHANG Zhi-yong

    1.School of Public Health，Guangxi Medical Universi-ty，Nanning 530021，China;2.Medical Scientific Research Center，Guangxi Medical University，Nanning 530021，China

    【Abstract】 Objective To compare the changes of protein expression in culture fluid of hepatocellular carcinoma SMMC-7721cells treated by antisense oligodeoxynucleotide(ASODN)and nonsense oligodeoxynucleotide(NODN)aimed at human telomerase RNA.Methods SELDI technology was used to detect the changes of protein expression.The control group was hepatocellular carcinoma SMMC-7721cells without any disposal.Results The results of SELDI showed that40proteins down-regulated and3proteins up-regulated in ASODN group ......