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Tec酪氨酸蛋白激酶的大鼠组织分布及参与的信号途径
http://www.100md.com 钟明贵, 李菲菲, 郑 红, 倪 芳, 余科科,
Tec酪氨酸蛋白激酶;肝再生;信号转导,钟明贵,郑红,倪芳,余科科,汪思应,李菲菲,钟明贵,通讯作者,Tissuedistributionandsi
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     钟明贵, 郑红, 倪芳, 余科科, 汪思应, 安徽医科大学基础医学院病理生理教研室 安徽省合肥市 230032

    李菲菲, 淮北市矿工总医院普外科 安徽省淮北市 235000

    钟明贵, 2003年安徽大学硕士研究生, 副主任医师, 主要从事肝再生分子调控的研究.

    国家自然科学基金资助, No. 30572206

    安徽省自然科学基金, No. 00044202, No. 01043709

    安徽省人才开发基金, No. 2002Z035

    通讯作者: 汪思应, 230032, 安徽省合肥市, 安徽医科大学基础医学院病理生理教研室. sywang@ahmu.edu.cn

    电话: 0551-5161129 传真: 0551-5167706

    收稿日期: 2006-05-09 接受日期: 2006-05-17

    Tissue distribution and signal transduction of Tec tyrosine kinase in rats

    Ming-Gui Zhong, Fei-Fei Li, Hong Zheng, Fang Ni, Ke-Ke Yu, Si-Ying Wang

    Ming-Gui Zhong, Hong Zheng, Fang Ni, Ke-Ke Yu, Si-Ying Wang, Department of Pathophysiology, Anhui Medical University, Hefei 230032, Anhui Province, China

    Fei-Fei Li, Huaibei Miner Hospital, Huaibei 235000, Anhui Province, China

    Supported by National Natural Science Foundation of China, No. 30572206; the Natural Science Foundation of Anhui Province, No. 00044202 and No. 01043709; and Human Resource Fund of Anhui Province, No. 2002Z035

    Correspondence to: Dr. Si-Ying Wang, Department of Pathophysiology, Anhui Medical University, Hefei 230032, Anhui Province, China. sywang@ahmu.edu.cn

    Received: 2006-05-09 Accepted: 2006-05-17

    Abstract

    AIM: To detect the tissue distribution of Tec tyrosine kinase in rats, and to investigate the possible signal transduction pathways in which Tec is involved.

    METHODS: The total RNA was extracted from the tissues of rat heart, liver, spleen, lung, brain, thymus gland and muscle. Northern blot was used to detect the expression of Tec RNA. Reporting gene plasmids and Tec expression vector were co-transfected into WBF-344 cells, and post-transfection cells were stimulated by hepatic growth factor (HGF). Then cells were lysed and the luciferace activity was measured.

    RESULTS: Northern blot suggested that Tec tyrosine kinase was highly expressed in the tissues of rat liver and kidney ......

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