【摘要】 目的 为人鼻腔黏液纤毛运输系统的研究提供科学有效的方法。方法 人鼻腔黏膜的组织块培养法，应用显微高速成像技术测量纤毛摆动频率。结果 在25℃，体外培养的人鼻腔黏膜纤毛摆动频率是10.39±1.80Hz；同一细胞任意两点纤毛摆动频率是相同的；同一来源的体外培养的钩突和下鼻甲的纤毛摆动频率是相同的。结论 钩突可成为今后人鼻腔纤毛系统的研究的主要标本来源。

    【关键词】 人鼻腔上皮细胞；纤毛摆动频率；钩突

    Ciliary beat frequency measurement of cultured human nasal epithelial cells

    SONG Xiao-hong，ZHANG Luo，HAN Demin，et al.Department of Otolaryngology Head and Neck Surgery，Beijing Tongren Hospital，Capital University of Medical Sciences，Beijing，100730，China

    【Abstract】 Objective To explore a scientifical method to research human mucociliary system.Methods Explant culture of human nasal epithelial cells. Ciliary beat frequency was measured by digital high-speed microscope. Results Ciliary beat frequency of cultured human nasal epithelial cells is 10.39±1.80 Hz at 25℃. Ciliary beat frequency of different cilia is equal on the same cell ......