菌核侧耳；_比色法；_三萜类_____菌核侧耳；

比色法测定菌核侧耳中三萜类成分的含量

http://www.100md.com 《时珍国医国药》 2006年第6期

菌核侧耳；,比色法；,三萜类,,菌核侧耳；,比色法；,三萜类,摘要,关键词,仪器与材料,2方法与结果,3讨论,参考文献

     摘要：目的对菌核侧耳中的三萜类成分进行含量测定。方法采用比色法，测定了菌核侧耳菌丝体和子实体三萜类化合物的含量，还优选了其显色条件，5%香草醛-冰醋酸最佳用量为0.2 ml，高氯酸最佳用量为1 ml。结果菌核侧耳子实体总三萜含量为1.16%，菌丝体为1.31%，RSD为1.05%。结论该方法简便、迅速可靠。

    关键词：菌核侧耳；比色法；三萜类

    The Content of Triterpenoids in Pleurotus tuber-regium(Fr.) Sing Determined by Colorimetry

    YU Zhijian, BAO Shuiming, LI Rongtong

    (Department of Biology, East China College of Science and Technology, Fuzhou, Jiangxi 344000, China)

    Abstract：ObjectiveTo determine the content of triterpenoids from mycelial and fruit body of Pleurotus tuber-regium(Fr.) Sing were determined by colorimetry.MethodsThe optimum volume of 5% vanillin-glacial acetic acid was 0.2 ml, and perchloric acid was 1 ml. ResultsThe results shaved triterpenoids composition of fruit body is 1.16%, the mycelium was 1.31%, RSD was 1.05%.ConclusionThis method is simple and convenient, fast and reliable. The Color Conditions were optimized.

    Key words：Pleurotus tuber-regium(Fr.) Sing; Colorimetry; Triterpenoids

    菌核侧耳Pleurotus tuber-regium(Fr.) Sing别名虎奶菌、核耳菇等 ......

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