板蓝根；_F022部位；_内毒素；_分子机理_

板蓝根F022部位抗内毒素分子机理研究

http://www.100md.com 《时珍国医国药》 2006年第6期

板蓝根；,F022部位；,内毒素；,分子机理,,板蓝根；,F022部位；,内毒素；,分子机理,摘要,关键词,1器材,2方法,3结果,4讨论,参考文献

     摘要：目的探讨板蓝根F022部位抗内毒素分子机理。方法内毒素定量检测法测定F022样品液对内毒素的破坏作用；研究样品液对内毒素致鼠巨噬细胞分泌炎性因子的抑制作用、对蛋白激酶MAPKp38活性的影响、对内毒素刺激鼠组织moesin mRNA分子表达及对内毒素诱导鼠体内TNFα,IL6和NO的抑制作用。结果1%F022对内毒素破坏率达到86.5%，F022能显著抑制内毒素刺激巨噬细胞分泌TNFα，IL6水平；抑制内毒素刺激蛋白激酶MAPKp38活性；降低内毒素刺激鼠肝、肾、脾组织moesin mRNA和体内TNFα，IL6和NO等炎性因子的表达。结论F022部位不仅直接破坏内毒素结构，且阻断内毒素引起的信号传导通路，从而抑制机体炎性因子的过度释放、抑制膜结构伸展刺突蛋白和丝裂原活化蛋白激酶的表达，从分子水平阐明了板蓝根中活性部位F022抗内毒素的分子机理。

    关键词：板蓝根； F022部位；内毒素；分子机理

    Studies on the Antiendotoxic Molecular Mechanism of F022 from Radix Isatidis

    LIN Aihua， LIU Yunhai， LIU Yiming

    (1.Second Affiliated Hospital of Guangzhou University of TCM, Guangzhou 510120,China; 2.Tongji Hospital, Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430030,China)

    Abstract：ObjectiveTo study the anti-endotoxic molecular mechanism of F022 from Radix Isatidis. MethodsThe content of F022pretreated ET was quantitatively determined with limulus test.The inhibition of TNFα and IL6 of murine peritoneal macrophages stimulated by LPS,the molecular expression of moesin mRNA, MAPK p38, TNFα,IL6 and NO in mice tissues induced by LPS were studied on the antiendotoxic mechanism of F022. ResultsIt was found that the ET reduction rate was 86.5%, if 1%F022was added to macrophages culture before addition of 50 ng·ml 1LPS.Production of TNFα, IL6 and MAPKp38 by macrophages were inhibited in vitro.F022 remarkably decreased the molecular expression of moesin mRNA in liver ......

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