【摘 要】 目的 探讨环磷酰胺对雄性小鼠生殖细胞毒性的影响。方法 雄性小鼠随机分为对照组与实验组,每组10只,实验组腹腔注射环磷酰胺溶液30 mg/(kg·d)，对照组腹腔注射生理盐水,连续5 d染毒,染毒后28 d取双侧睾丸和附睾，计数小鼠精子畸形率和生殖细胞微核发生情况,评价环磷酰胺对小鼠的生殖毒性作用。结果 腹腔注射环磷酰胺实验组精子畸形率与对照组相比显著升高(P<0.05)；实验组生殖细胞微核率与对照组相比亦显著升高(P<0.05)。结论 小鼠腹腔注射环磷酰胺对雄性小鼠生殖细胞有损害作用。

    【关键词】 环磷酰胺；精子;睾丸;生殖细胞;微核

    Effect on Testicular Germ Cell after Exposure to Cyclophosphamide in Mice JIA Qingjun*,LIU Tianpeng,GUO Kuiliang,et al.*Bethune military medical college,Shijiazhuang 050081,China

    【Abstract】 Objective To study the reproductive damage of sperm and testicular germ cell induced with cyclophosphamide (CP) in male mice. Methods 20 mice were randomly divided into two groups.Physiological saline was injected in control group,CP 30 mg/(kg·d) was injected in experimental group. After 28 days ......