己酮可可碱·间质胶原酶·金属蛋白酶1组织抑制剂·转化生长因子

己酮可可碱对大鼠胰腺纤维化及TGFβ1、αSMA、MMP1、TIMP1表达的影响

http://www.100md.com 《中国普通现代外科进展》 2006年第4期

己酮可可碱·间质胶原酶·金属蛋白酶1组织抑制剂·转化生长因子β·α平滑肌肌动蛋白·胰腺炎·纤维化·大鼠,Wistar,,己酮可可碱·间质胶原酶·金属蛋白酶1组织抑制剂·转化生长因子β·α平滑

     【摘要】目的：探讨己酮可可碱(PTX)对大鼠胰腺纤维化及TGFβ1、αSMA、MMP1、TIMP1表达的影响。方法：通过胰管内注射2%三硝基苯磺酸的方法制备大鼠胰腺纤维化模型。随机分为对照组、模型组、PTX干预组。PTX干预组于术后第2天给予PTX腹腔注射，6mg/(kg·d)，4周后收集各组胰腺标本。免疫组化SABC法检测各组胰腺组织中α平滑肌肌动蛋白(αSMA)、转化生长因子β1(TGFβ1)、基质金属蛋白酶1(MMP1)、金属蛋白酶组织抑制物1(TIMP1)的表达同时做组织胶原纤维染色。部分胰腺组织液氮冻存，以Western blot方法检测αSMA的表达。结果：模型组发生胰腺纤维化，PTX干预组有轻度纤维化。PTX干预组αSMA、TGFβ1、TIMP1的表达均低于模型组(P<0.01)。MMP1的表达无明显差异。PTX干预组胶原面积百分比明显低于模型组(P<0.01)。结论：PTX抗大鼠胰腺纤维化的作用可能与其降低TGFβ1、αSMA、TIMP1表达有关。

    【关键词】己酮可可碱·间质胶原酶·金属蛋白酶1组织抑制剂·转化生长因子β·α平滑肌肌动蛋白·胰腺炎·纤维化·大鼠，Wistar

    Effect of pentoxifylline on the expressions of TGFβ1,αSMA,MMP1 and TIMP1 in rat pancreatic fibrosis

    WANG Dongsheng1,LIU Qian2

    1Department of Interventional Radiology,Coal General Hospital(Beijing 100028,China)

    2Department of Digestion,Shandong Provincial Qianfoshan Hospital(Jinan 250014,

    China)

    【ABSTRACT】Objective:To observe the effect of pentoxifylline on pancreatic fibrosis and the expressions of TGFβ1,αSMA,MMP1 and TIMP1 in rat.Methods:The injection method of 2% TNBS through rats’s pancreatic duct was undertaken to set up the animal model of pancreatitis,Mice of interventional group were treated with PTX,and the samples of pancreas were collected after 4 weeks treatment for evaluating the expression of TGFβ1,αSMA,MMP1 and TIMP1 by using immunohistochemistry technique.Masson staining of pancreas were undertaken.The expression of αSMA in some samples were evaluated by using Western blot.Results:Pancreas fibrosis was observed in model group rats and there were no significant pancreas fibrosis in PTX interventional group.PTX interventional significantly reduced the content of TGFβ1,αSMA,and TIMP1 compared to the model(P<0.01),while that of MMP1 was unchanged in PTX interventionalgroup.Pancreas fibrosis proportion was also reduced in PTX interventional group(P<0.01).Conclusion:PTX could inhibit the formation of pancreas fibrosis in rats,which might relate to the decreasing expression of TGFβ1,αSMA,TIMP1. ......

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