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反相高效液相色谱法测定山楂中的有机酸
http://www.100md.com 《分析化学》 2006年第7期
反相高效液相色谱,山楂,有机酸,,反相高效液相色谱,山楂,有机酸,摘要,关键词,1引言,2实验部分,3结果与讨论,References
     摘 要 采用Hypersil ODS2(4.6 mm×250 mm,5 μm)色谱柱,以磷酸盐缓冲溶液(pH=2.6)为流动相,流速0.8 mL/min,检测波长210 nm,建立了山楂中柠檬酸、苹果酸等有机酸的高效液相色谱分析方法。其中柠檬酸和苹果酸的线性范围分别为0.50~50.04 μg、0.10~10.00 μg;3个浓度水平的回收率分别为95.5%~966%、100.2%~103.7%,RSD分别为0.11%~0.38%、0.41%~2.53%;检出限分别为0.04 μg、0.03 μg(S/N=3)。测定了5种不同产地山楂果肉及山楂核中的柠檬酸、苹果酸的含量。所建立的方法快速﹑灵敏度高、重复性好,可用于山楂中有机酸的测定。

     关键词 反相高效液相色谱,山楂,有机酸

    Analysis of Organic Acids in Hawthorn by Reversed Phase High Performance Liquid Chromatography

    Jin Gaowa, Zhang Feifang*, Xue Xingya, Xu Qing, Liang Xinmiao

    (Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023)

    Abstract Hawthorn is a traditional Chinese medicine and fruit. It contains numerous organic acids. It is reported that organic acids have the ability to cure indigestion and have many other functions. A quantitative reversedphase high performance liquid chromatography method using diode array detector has been developed for the determination of citric acid and malic acid in hawthorn. A Hypersil ODS2 column was used with phosphate buffer solution (pH=2.6) as the mobile phase at a flow rate of 0.8 mL/min. The column temperature was 25℃ and the UV detection wavelength was 210 nm. The calibration curve for citric acid and malic acid was in good linearity in the range of 0.50-50.04 μg with the regression equation Y=78.2X-0.08 ......

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