氧化苦参碱抑制人肝癌细胞EGF和EGFR表达的研究
氧化苦参碱·表皮生长因子·受体,表皮生长因子·癌,肝细胞,,氧化苦参碱·表皮生长因子·受体，表皮生长因子·癌，肝细胞,1材料和方法,2结果,3
【摘要】目的：探讨氧化苦参碱对人肝癌细胞EGF及EGFR表达的影响。方法：将肝癌细胞株BEL7402在37℃、5%CO2条件下培养、传代。按氧化苦参碱浓度分为1000μg/ml、500μg/ml、250μg/ml 3组，对照组不加氧化苦参碱，每组设6个平行孔，每孔加入细胞1×105/ml，培养24h、48h和72h，分别进行EGF及EGFR的免疫组化染色。结果：不同浓度的氧化苦参碱均抑制EGF和EGFR在人肝癌细胞的表达，随着培养时间的延长，EGF和EGFR表达阳性率下降显著，在1000μg/ml组24h、48h和72h的EGF和EGFR阳性率均较500μg/ml组和250μg/ml组同时段的阳性率下降显著(P0.05,P0.01)。500μg/ml组和250μg/ml组同时段的阳性率无明显差异。结论：氧化苦参碱对EGF和EGFR的抑制途径可能是其抗肝癌机制之一。【关键词】氧化苦参碱·表皮生长因子·受体，表皮生长因子·癌，肝细胞
Inhibiting effect of oxymatrine on the expression of EGF and EGFR in human hepatocellular carcinoma cells in vitro
WANG Wenqi,LIU Qian,XU Hanfeng,SHI Changwen,WU Hongwen
Deparement of Gastroenterology,Shandong Qianfoshan Hospital (Jinan 250014,China)
【ABSTRACT】Objective:To investigate the effect of oxymatrine on EGF and EGFR expression in human hepatocellular carcinoma cells,BEL7402.Methods:BEL7402 cells were cultured under the condition of 37℃,5%CO2.BEL7402 cells were treated by oxymatrine with different concentration,including 1000μg/ml,500μg/ml,250μg/ml,and 0μg/ml,and the observation period was from 24h to 72h.Four groups were divided.Each group contains six wells.There are 1×105/ml cells in each well.The expression of EGF and EGFR were observed with immunohistological technique.Results:The expression of EGF and EGFR were suppressed by oxymatrine with different concentration and variable durations.In Group A(1000μg/ml),the expression of EGF and EGFR at 24h,48h and 72h decreased more significantly than Group B(500μg/ml),and Group C(250μg/ml) (P0.05,P0.01).There were no significant difference between EGF and EGFR expression at the different treating durations in Group B and Group C.Conclusion:Oxymatrine can play a certain role in treatment of liver cancer through inhibiting the expression of EGF and EGFR. ......
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