反相高效液相色谱法测定阿德福韦酯脂质体药物含量
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徐速，邓意辉，吴红兵，张玲玲 (沈阳药科大学 药学院，辽宁 沈阳 110016)
摘要：目的 建立测定阿德福韦酯脂质体中药物含量的方法。 方法 采用Diamonsil C18柱 (4.6 mm×200 mm, 5 μm)，流动相为0.025 mol·L-1磷酸二氢钾-乙腈(体积比为 65:35 )，流速为 1.0 mL·min-1，检测波长为260 nm，柱温为室温。结果 在上述色谱条件下阿德福韦酯与辅料、溶剂及有关物质分离度均符合要求，在40 ~ 800 mg·L-1内呈良好的线性关系(r = 0.999 9, n = 6)，阿德福韦酯平均回收率为100.06 %，RSD为0.35 % (n = 3)。结论 HPLC法可用于阿德福韦酯脂质体含量测定。
关键词：药剂学；阿德福韦酯；脂质体；反相高效液相色谱法；含量测定
中图分类号：R94 文献标识码：A
contents
Determination of adefovir dipivoxil in liposomes by RP-HPLC
XU Su; DENG Yi-hui; WU Hong-bing;ZHANG Ling-ling
(School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China)
Abstract: Objective To establish a RP-HPLC method for determination of adefovir dipivoxil in liposomes. Methods The chromatographic separation was performed with a Diamonsil C18 column (4.6 mm×200 mm, 5 μm) at room temperature, and the mobile phase was 0.025 mol·L-1 phosphate buffer–acetonitrile(65∶35, V/V), the flow rate was 1 mL·min-1. Adefovir dipivoxil was detected at 260nm. Results Good linearity over the concentration range of 40~800 mg·L-1 (r = 0.999 9, n = 6) was obtained in this experiment. The average recovery was 100.06 %. The RSD was 0.35 %. Conclusion This method is simple, accurate and applicable for the determination of adefovir dipivoxil in liposomes.
Key words: pharmaceutics; adefovir dipivoxil; liposomes; RP-HPLC; content determination
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