尾静脉液压法注射转hIL-10在大鼠肝组织中的表达
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成 峰, 王学浩, 肇 毅, 黄 蓬
尾静脉液压法;人类白细胞介素10;SB转座元;大鼠,成峰,王学浩,肇毅,黄蓬,通讯作者,Hydrodynmics-baseddeliveryofhu
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成峰, 王学浩, 南京医科大学第一附属医院肝脏移植中心 江苏省南京市 210029
肇毅, 南京医科大学第一附属医院普通外科 江苏省南京市 210029
黄蓬, 南京医科大学动物实验中心 江苏省南京市 210029
通讯作者: 成峰, 210029, 江苏省南京市广州路300号, 南京医科大学第一附属医院肝脏移植中心. docchengfeng@sohu.com
电话: 025-83718836-6476 传真: 025-83672106
收稿日期: 2006-05-05 接受日期: 2006-05-29
Hydrodynmics-based delivery of human interleukin-10 gene in rats
Feng Cheng, Xue-Hao Wang, Yi Zhao, Peng Huang
Feng Cheng, Xue-Hao Wang, Liver Transplantation Center, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, Jiangsu Province, China
Yi Zhao, Department of General Surgery, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, Jiangsu Province, China
Peng Huang, Animal Experiment Center, Nanjing Medical University, Nanjing 210029, Jiangsu Province, China
Correspondence to: Feng Cheng, Center of Liver Transplantation, the First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing 210029, Jiangsu Province, China. docchengfeng@sohu.com
Received: 2006-05-05 Accepted: 2006-05-29
Abstract
AIM: To study hydrodynmics-based delivery of human interleukin-10 (hIL-10) transferred with Sleeping Beauty (SB) transposon system in rats.
METHODS: All the rats were injected with 20 mL Rinegers solution within 10-12 s via the tail vein. The rats were divided into the following groups according to different treatments: Ringers control; 100 μg empty liposome DOTAP; 100 μg liposome DOTAP + 50 μg pT-hIL-10; 50 μg pT-hIL-10 + 5 μg pCMV-SB (n = 6, in each group). The levels of alanine aminotransferase (ALT) were measured in each group, and the concentrations of hIL-10 in the serum were examined by enzyme-linked immunosorbent assay (ELISA) on postoperative day 1, 4, 7, 14 in transferred gene groups, respectively. The expression of hIL-10 mRNA was detected by reverse transcription polymerase chain reaction (RT-PCR) ......
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