当前位置: 首页 > 期刊 > 《第四军医大学学报》 > 2006年第13期
编号:11131816
生物介质中MN9202检测方法的建立及其应用
http://www.100md.com 《第四军医大学学报》 2006年第13期
MN9202;反相高效液相色谱;生物介质,,MN9202;反相高效液相色谱;生物介质,0引言,1材料和方法,2结果,3讨论,【参考文献】
     Establishment of a method of determining MN9202 in biomedium and its application

    RAN YuHua, ZHOU SiYuan, LIU XinYou, TENG ZengHui, YANG RunTao, YANG ZhiFu, MEI QiBing

    Department of Pharmacology, School of Pharmacy, Fourth Military Medical University, Xian 710033, China

    【Abstract】 AIM: To establish a method to determine 2,6Dimethyl4(3nitropheny1)1,4 dihydropyridine3carboxylic acid dimethylester5carboxylic acid pentyl ester (MN9202) in biological medium by reversephase highperformance liquid chromatography (RTHPLC). METHODS: The specimen containing the lysate of Caco2 cells and rat plasma were extracted with ether. The organic phase was removed into the tubes and then evaporated in ventilation cabinet. The residue was dissolved in 50 μL of methanol, then 20 μL solution was drawn and detected by RTHPLC. RESULTS: MN9202 could be disassociated from interference in the certain mobile phrases, the regression equation of the standard curve to detect the cell samples was Y=0.0048X+0.0592, r=0.9997, n=7 (P<0.01), the linear range was 20-500 mg/L; The standard curve to detect MN9202 in the plasma was worked out, and the regression equation was Y=0.0058X+0.1468, r=0.9998, n=7 (P<0.01), the linear range was 20-500 mg/L. CONCLUSION: The analytical method was convenient, stable, sensitive and accurate. It could be applied to the determination of MN9202 in biological medium. ......

您现在查看是摘要页,全文长 7748 字符