NS-398对HGF诱导的肝癌细胞株MMP-7, MMP-9, TIMP-1表达的影响
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殷积彬, 刘铁夫, 孟祥宁, 赵丽莉, 刘晓川
COX-2抑制剂;肝细胞生长因子;基质金属蛋白酶;基质金属蛋白酶抑制剂;侵袭力;肝癌细胞株,NS-398对HGF诱导的肝癌细胞株MMP-7,MMP-9,TIMP-1表达的影响,殷积彬,刘铁夫,赵丽莉
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殷积彬, 哈尔滨医科大学附属第二医院消化科 黑龙江省哈尔滨市 150086
刘铁夫, 赵丽莉, 刘晓川, 哈尔滨医科大学附属第四医院消化科 黑龙江省哈尔滨市 150001
孟祥宁, 哈尔滨医科大学遗传教研室 黑龙江省哈尔滨市 150086
殷积彬, 1998年哈尔滨医科大学学士, 2003年哈尔滨医科大学附属第四医院消化科博士生, 主要从事肝胆疾病的诊断与治疗.
通讯作者: 刘铁夫, 150001, 黑龙江省哈尔滨市, 黑龙江省哈尔滨医科大学附属第四医院消化科.TFLiu@mail.csoft.com.cn
电话: 0451-82576696
收稿日期: 2006-06-06 接受日期: 2006-06-30
Effect of NS-398 on matrix metalloproteinase expression in hepatocyte growth factor-induced HepG2 cell line
Ji-Bin Yin, Tie-Fu Liu, Xiang-Ning Meng, Li-Li Zhao, Xiao-Chuan Liu
Ji-Bin Yin,Department of Gastroenterology, the Second Affiliated Hospital of Harbin Medical University, Harbin 150086, Heilongjiang Province, China
Tie-Fu Liu, Li-Li Zhao, Xiao-Chuan Liu,Department of Gastroenterology, the Fourth Affiliated Hospital of Harbin Medical University, Harbin 150001, Heilongjiang Province, China
Xiang-Ning Meng,Department of Genetics, Harbin Medical University, Harbin 150086, Heilongjiang Province, China
Correspondence to:Tie-Fu Liu, Department of Gastroenterology, the Fourth Affiliated Hospital of Harbin Medical University, Harbin 150001, Heilongjiang Province, China. TFLiu@mail.csoft.com.cn
Received:2006-06-06 Accepted:2006-06-30
Abstract
AIM: To investigate the effect of the selective cyclooxygenase-2 (COX-2) inhibitor NS-398 on the expression of matrix metalloproteinases (MMPs) and tissue inhibitor of metalloproteina-ses (TIMPs) in liver cancer cell line induced by hepatocyte growth factor (HGF), and to explore the possible mechanism of NS-398 in depressing tumor cell invasiveness.
METHODS: HGF-induced liver cancer cells HepG2 were treated with different concentrations of NS-398 (0, 20, 40, 60, 80 µmol/L). The expression of MMP-7, MMP-9 and TIMP-1 inside the cells, the contents outside of the cells and the levels of mRNA were observed by immunohistochemistry staining, enzyme-linked immunosorbent assay (ELISA), and reverse transcription-polymerase chain reaction (RT-PCR), respectively ......
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