二异丙酚;心肌再灌注损伤；半胱天冬酶12;凋亡_

异丙酚对兔缺血/再灌注损伤心肌半胱天冬酶12的影响

http://www.100md.com 《河北医药》 2006年第6期

二异丙酚;心肌再灌注损伤；半胱天冬酶12;凋亡,,二异丙酚;心肌再灌注损伤；半胱天冬酶12;凋亡,【摘要】,【关键词】,1材料与方法,2

     【摘要】 目的观察异丙酚对兔缺血/再灌注损伤心肌半胱天冬酶12(caspase12)的影响，从内质网角度探讨异丙酚对减少缺血/再灌注损伤心肌细胞凋亡的机制。方法健康雄性新西兰大白兔24只，体重2.1～2.3 kg。随机分为假手术组(Sham组)、缺血/再灌注组(I/R组)和异丙酚组(P组)(每组8只)。术中胸膜破裂和死亡者不纳入实验,再按照随机原则在相同条件下予以补齐。实验结束后取左心室缺血/再灌注区心肌组织，免疫组织化学方法检测caspase12、半胱天冬酶3(caspase3)，用Motic 6.0图像分析系统进行处理,比较各观测指标平均光密度值(Average optical value AO值)；用流式细胞检测技术(flow cytometry,FCM)及DNA原位末端缺口标记法(TUNEL法)检测缺血/再灌注区心肌细胞凋亡。结果 Sham组caspase12、caspase3低表达;FCM检测有少量凋亡早期细胞，TUNEL染色见少量阳性细胞；与Sham组比较，I/R组和P组caspase12、caspase3表达增加(P<0.05)，FCM检测和TUNEL染色见凋亡细胞数增加(P<0.05)；与I/R组比较，P组Caspase12、Caspase3表达下降(P<0.05)，FCM和TUNEL染色见凋亡心肌细胞数减少(P<0.05)。结论抑制caspase12介导的caspase级联反应性凋亡途径的激活是异丙酚减少缺血/再灌注损伤心肌细胞凋亡的机制之一。

    【关键词】 二异丙酚;心肌再灌注损伤；半胱天冬酶12;凋亡

    The effects of propofol on the caspase cascade reaction induced by caspase12 in myocardial ischemiareperfusion injury

    DONG Zhenming，REN Jianjun.

    Department of Anesthesiology,The Second Hospital of Hebei Medical University,Shijiazhuang 050000

    【Abstract】 Objective To investigate the effect of propofol on the caspase12 in ischemiareperfusion myocardium,to elucidate the protective mechanism of propofol on myocardial tissue underwent ischemiareperfusion in rabbit heart.Methods Twentyfour healthy male white rabbits (4 months of age) weighting 2.1～2.3 kg were randomly divided into sham operation group,ischemiareperfusion group and propofol group.The rabbits were anesthetized with 3% pentobarbital 30 mg/kg,to make the model of ischemiareperfusion injury according to the referent literatures. The propofol (2.5 mg·kg·-1·h-1) was infused through the vein at the edge of ear,then infusing it at the dose of 5 mg·kg-1·h-1 continuesly to the end of experiment.The tissues of ischemiareperfusion region were taken to detect the expression of caspase12,caspase3 by immunohistochemistry and to analyze them with Motic 6.0 image analysis system,and to detect the I/R myocardial apoptosis by flow cytometry and TUNEL staining methods.Results The caspase12 and caspase3 expresses in a low level,a few apoptotic cells were detected by FCM and the TUNEL staining in sham operation group.As compared with that in sham operation group,the expression of caspase12 and caspase3 in I/R group and propofol group were higher (P<0.05);the apoptotic cells detected by FCM and TUNNEL methods were significantly increased,as compared with those of sham operation group (P<0.05).As compared with those of I/R group,the levels of caspase12 and caspase3 in propofol group were lower than that of I/R group,the percentage of apoptotic cells was lower than that of I/R group (P<0.05).Conclusion Propofol can decrease the number of apoptotic cells of the ischemiareperfusion myocardium,inhibit the caspase cascade reaction induced by caspase12,which is one of the protective mechanisms of propofol. ......

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